Gram-Positive Bacilli (Rods) and Diseases

Gram-positive bacteria retain the color of the primary stain (crystal violet) in the Gram staining procedure and appear as purple/violet under a light microscope. These bacteria have a cell wall containing a thick layer of peptidoglycan. On the basis of cell morphology, Gram-positive bacteria are divided mainly into two groups, “Gram-positive cocci” and “Gram-positive bacilli”.

Bacillus anthracis in Gram stain

Gram-positive bacilli are a diverse group of bacteria which can further be classified into various subgroups based on their characteristics such as

  1. Endospore forming ability: Bacillus and Clostridia are spore-forming rods while Listeria and Corynebacterium are not.
  2. Gaseous (oxygen requirements):  Bacillus species are obligate aerobes whereas Clostridium species are obligate anaerobes.

Some of the Gram-positive rods have a unique shape based on the position of spores, presence of branches etc. Some of the typical morphological shapes of the Gram-positive bacilli are summarized in the table below:

Organisms Typical Shape Remarks
Actinomyces Irregular/branching  
Corynebacteria Club-shaped In Gram stain, these bacteria appear as V-in Y-shaped arrangements or in clumps that resemble Chinese letters.
Clostridium tetani Drum stick or tennis racket appearance Due to the presence of spherical and terminal spore.
Nocardia Branching filaments In Gram stain, these bacteria stain irregularly and appear beaded.

Gram-positive bacilli are responsible for “classical” diseases such as anthrax, diphtheria, and listeriosis and also for newer syndromes, particularly in immunocompromised individuals.

Some of the diseases caused by gram-positive bacilli are as follows:

Organism (s) Typical agent Disease (s)
Actinomyces Actinomyces israelii Actinomycosis
Corynebacteria Corynebacterium diphtheriae Diphtheria
Listeria   Listeria monocytogenes   Listeriosis, neonatal meningitis, sepsis etc.
Clostridium Clostridium perfringens Gas gangrene (clostridial myonecrosis)
  Clostridioides difficile Diarrhea and colitis
  Clostridium tetani Tetanus
  Clostridium botulinum Botulism
Bacillus Bacillus cereus Foodborne diseases
  Bacillus anthracis Anthrax
Erysipelothrix Erysipelothrix rhusiopathiae Erysipeloid
Nocardia Nocardia asteroides Nocardiosis
Cutibacterium Cutibacterium (formerly Propionibacterium) acnes Acne
Eubacterium Various species Minor clinical importance
Mycobacterium Mycobacterium tuberculosis Tuberculosis
  Mycobacterium leprae Leprosy

Non-pathogenic Gram-positive rods

Lactobacillus: Lactobacillus is an important microbiota of the birth canal and helps to prevent the growth of many potential pathogens. They are mainly used in the manufacture of fermented dairy, sourdough, meat and vegetable foods or used as probiotics.

Streptomyces:  Streptomyces is a Gram-positive bacteria which shape resembles filamentous fungi. Streptomyces is known for its ability to produce bioactive secondary metabolites such as antifungals, antivirals, and mainly antibiotics. Today, nearly 80% of the antibiotics are sourced from the genus Streptomyces such as penicillin, streptomycin, gentamicin, linezolid, tetracycline, etc.

About Acharya Tankeshwar 444 Articles
Hello, thank you for visiting my blog. I am Tankeshwar Acharya. Blogging is my passion. I am working as an Asst. Professor and Microbiologist at Department of Microbiology and Immunology, Patan Academy of Health Sciences, Nepal. If you want me to write about any posts that you found confusing/difficult, please mention in the comments below.

3 Comments

Do you have any queries? Please leave me in the comments section below. I will be happy to read your comments and reply.

This site uses Akismet to reduce spam. Learn how your comment data is processed.