Giemsa stain is a type of Romanowsky stain, named after Gustav Giemsa, a German chemist who created a dye solution. It was primarily designed for the demonstration of malarial parasites in blood smears, but it […]
Gram staining requires the use of different chemical reagents which can be purchased in ready to use forms from commercial suppliers or gram stain reagents can be prepared at the laboratory by mixing different chemicals […]
The simple stain can be used as a quick and easy way to determine cell shape, size and arrangements of bacteria. True to its name, the simple stain is a very simple staining procedure involving […]
Two methods, thick and thin blood smear microscopy are regarded as the “gold standard” for the diagnosis of malaria. Quantitative Buffy Coat is another direct and rapid test for diagnosis of malaria. It is based […]
Acridine orange is a dye that intercalates or binds with the nucleic acid ( either DNA or RNA) present in organisms and fluoresce to emit various colors that help in differentiation of cellular organells. This […]
Albert stain is a type of differential stain used for staining high-molecular-weight polymers of polyphosphate known as metachromatic granules or volutin granules found in Corynebacterium diphtheriae. Metachromatic granules are also found in Yersinia pestis, and […]
When vegetative cells of certain bacteria such as Bacillus spp and Clostridium spp are subjected to environmental stresses such as nutrient deprivation, they produce metabolically inactive or dormant form-endospore. Formation of endospores circumvent the problems […]
Auramine-Rhodamine Fluorochrome staining also known as “Truant method of staining”, is used to visualize Acid fast bacilli (AFB). Ziehl-Neelsen (hot), Kinyoun (cold) are still widely used methods in developing countries. CDC recommends fluorochrome staining for detecting […]
Gram staining method, the most important procedure in Microbiology, was developed by Danish physician Hans Christian Gram in 1884. Gram staining is still the cornerstone of bacterial identification and taxonomic division. This differential staining procedure separates […]
Bacteria are easier to detect in broth culture if the preparation is stained using toluidine blue 0.5% w/v, rather than stained by the Gram staining technique. Procedure of Toluidine Blue Staining Make a smear of […]
Most motile bacteria possess flagella, the shape, number and position of which are important characteristics in the differentiation of genera and species identification. Read more about structure and importance of bacterial flagella clicking this link. […]
Ziehl-Neelsen (ZN) method of Acid Fast staining technique is used to stain Mycobacterium species including M. tuberculosis, M. ulcerans, and M. leprae and nontuberculous mycobacteria (NTM). Detection of acid-fast bacilli (AFB) in stained and acid-washed […]
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