Gram-positive bacteria retain the color of the primary stain (crystal violet) in the Gram staining procedure and appear as purple/violet under a light microscope. These bacteria have a cell wall containing a thick layer of […]
This post was most recently updated on December 14th, 2019Tinsdale agar base medium was developed by Tinsdale for the selective isolation and differentiation of C. diphtheriae from diphtheroids. Billings later modified the medium improving its […]
This post was most recently updated on December 14th, 2019Traditionally, Cystine Trypticase Agar (CTA) supplemented with a 1% carbohydrate solution was used to determine acid production by Neisseria and related species. This test is no […]
Furazolidone (Furoxone) disk test is performed as a disk susceptibility procedure using commercially available furazolidone disks. Staphylococci are inhibited by furazolidone but micrococci and related species are resistant. This is one of the commonly used […]
Biological safety cabinet (BSC) is an engineering control intended to protect laboratory workers, laboratory environment and work materials from exposure to infectious or biohazardous aerosols and splashes. Such aerosols and splashes may be generated while […]
Amies transport medium is a widely used and effective semisolid medium for the transportation of swab specimens to the microbiology laboratory. Placing swabs in a moist container or transport medium prevents drying and death of […]
This post was most recently updated on November 30th, 2019The lac operon is a well-known example of an inducible gene network which regulates the transport and metabolism of lactose in Escherichia coli. It encodes the […]
Microbes are loosely classified into several groups based on their preferred temperature ranges. Every organism has a range of permissive growth temperature. Unlike pH or osmolarity, microbes cannot regulate their temperature i.e. their internal temperature […]
This post was most recently updated on January 21st, 2020Staphylococcus aureus is a notable human pathogen for a variety of infections; suppurative (pus-forming) infections, systemic illness and toxinoses. S. aureus has an extraordinary repertoire of […]
Glycopeptide antibiotics act primarily by inhibiting cell wall synthesis of bacteria but functions differently from beta-lactam antibiotics. Vancomycin is the most important and commonly used glycopeptides, although teicoplanin is also available. Glycopeptides are active against […]
Automated blood culture systems are the primary choice for the detection of pathogens from blood specimens. Instead of manually read broth-based conventional methods, laboratories use continuously monitored, automated blood culture systems for the processing of […]
Ames test devised by a scientist “Bruce Ames” and colleagues at the University of California in Berkeley, is a method for evaluating mutagenic effects of chemicals, drugs or implant device. This test is used to […]
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