Welcome to Microbeonline.com. I am Tankeshwar Acharya. I am as working as a Assistant Professor and Microbiologist at Department of Microbiology and Immunology,  Patan Academy of Health Sciences, Lalitpur, Nepal.about me

My life partner Nisha Rijal is supporting me for the maintenance and moderation of this website.  She is working as a  Microbiologist in National Public Health Laboratory , National reference laboratory of government of Nepal.

I have completed my Masters (M.Sc)  in Medical Microbiology with distinction from Tribhuvan University Nepal, which is the largest and oldest University of Nepal.

During off hours at Patan Academy of Health Sciences, I am guiding students of Masters in Medical Microbiology  at  National College.

Blogging is my passion, I started blogging in 2008.   I have  co-founded Nepal’s first comprehensive educational portal: edusanjal.com, which is now Nepal’s No. 1 Educational website and helps millions of  Nepalese students about college/course guidance and provides them regular updates about scholarships, educational events, education news etc for which in 2011 we got World Summit Youth Award for the Category Education for All

I am planning for my PhD study. My research interest includes but not limited to Host Pathogen-interaction, Immune defense mechanism, Microbial drug resistance etc.

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    • Sir , why don’t you write a book ?
      I request you to write one for us , it will be worthreading for UG students !

      • Thank you so much Vishal for your inspiring comment. Will think about this in future when i truly believe that i can give more values to students.

  1. Tankeshwar,

    You have a stellar website! Thanks so much for providing a great resource for other microbiology students.


  2. Dear basem muammar and Donovvan, thank you so much for your inspiring comments. I will try my best to make it useful website.

  3. what an awesome detailed website and information. I teach hair care and this is phenomenal info for my student to read and study and
    use as reference and its easy to understand thanks…

  4. Hlo. I sonia garg human genetics student i have to work on micronucleus assay test on bone marrow samples of leukemia patient.
    I am working on fluorescent microscope. Can you plz guide what kind of fluorescent dye will be used and staining time and much more about this. Thank you

  5. Doing a great job for students and teachers as well.
    This site is surely amazing.
    I wanted to tell I’m was a average student in the starting of 2nd year until i found this site, now my mates are surely jealous of me and sshhhh.. I don’t want to tell them my secret.
    Thanks you.
    Also please make it simplied for the diagnosis as..
    O- oraganism
    P- pathogenicity

    For each and every bacteria. Bacteriology.
    It would be great help!

    Also do simplified Virology.

    Thank you ACharya. ???? keep the good work!

    • Dear Faisal
      Thank you so much for your inspiring comment and this happiness. I am feeling awesome after knowing my work is improving your academic performance. I will try my best to implement the suggestion you have provided. Read well, keep in touch and keep suggesting.

  6. Hi,hello !
    Tankeshwar, My name is Cristiane and I don’t know if I am doing right to ask you this, I am not anstudent any more, I am just a house wife.
    I search the internet for answers but I never found any, so I am risking ask you, if you think it is not aproprieted just disregard it an do not take me wrong, sorry.
    My question is about garlic and mold.
    I am very fearful of coack roaches, but I hate poisoning my house too, so I discouver that the roaches hate garlic, than I got lots of glass botles with large openings and I put in it minced garlic and water, I put around the house in corners and near to places I’ve seen roaches( I’ve traveled and my husband toke care of the house by his self , he hate clean the kitchen) .
    The thing is , grow on top of the garlic, some very weird black mold, not all the botles but some of them, I am puzled because I never saw it before, it kind of foat on the water and under it are some pieces of the minced garlic.
    This mold is so thick loks like leather, black leather or black mushrooms.
    Can you tell me what is this ?
    I am sorry if it is not right, I’ve subscribed to your site but some thing went wrong and I just got this place to write.
    Thank you very much and congratulations for all you achievements !
    Cristiane J. Ferreira

    • Dear Cristiane
      Thank you so much for your valuable time and sending us this unique problem/comment to solve. I read your comment and i liked your enthusiasm and the efforts you have done to get rid of cockroaches. Instead of using pesticides, you are trying something organic, that sounds great. Did you get rid of cockroaches? Regarding the growth of fungi, there are various types of fungi which can grow on such conditions and can give black colored colonies. They may be any of
      Aspergillus,Penicillium, Fusarium or Cladosporium or any others too. Mycologist have to study its colony morphology, pigementation, arrangements of hyphae and spores (by microscopy) to identify exactly the name of the mold/fungi involved in your case. Sorry, i could not give you exact answer, if you are keen to know that species, i request you to visit nearby laboratory where fungal culture facilities is available, they will help you in this regard. Wishing you great time ahead,thank you for the question and the wish.

  7. Good day sir,
    my name is michael, your website has been of great importance to me. it has really developed my knowledge about the practical aspect of microbiology.
    However, i have two questions that has been bothering me since few weeks ago. i’ll be glad if you can help me out or probably share me a link that would be of help.
    1. what are the roles of plasmids in bacteria virulence and antibiotic resistance. (2 pages)
    2. what are the methods of eliminating plasmids. (1 page)
    answers are needed urgently. thank you.

  8. I came across your site while designing a simplified E.coli test. I like your site. Want to know iof you are interested in broadening and deepening the contents in the site. I am interested in having the contents in English and Chinese.
    You expressed an interest in pursuing a Ph.D degree—would you like to do this in China?
    You can find out a little more information on me by a Google search: “Peter Chun WHO WTO, WIPO 2014”

  9. Hi,

    could you please provide a reference for the gram staining procedure page. For example, the textbook where you got this information from?

    Many thanks

  10. I love your website but in the upper right corner, the word ‘mycology’ takes up all the text box used to do a search. Can you reformat it somehow?

  11. I just want to say that this is a GREAT website with so much information; but I also very much respect what you’re doing here with the online marketing and strategies for generating blogging income. I want to create something like this (but for a language-related blog) and actually – I hope we can connect in the (possibly near) future so that maybe we could link each other.. My site isn’t up yet because I’m still company freelancing and I’ve gotten bombarded with papers recently. Actually, YOUR website helped me with one of my papers! When I gain some momentum, I will get back to you. Love the passion and dedication to microbes here. I never really thought microbes were fun until I started researching them..

  12. Hi Mr. Tankeshwar,
    There is a student looking for FTA-ABX (Fluorescent Treponemal Antibody Absorption) Test kit. Please advise if you can offer this product. She needs around 500 tests. Please details with prices via email.
    Thanks and best regards,

    • Dear Abbas
      Sorry we do not supply such test KITS. This blog is only for the educational purpose and not a site maintained by the supplier or manufacturer of such kit.

  13. Hi, pls sir I’m a student of microbiology I’ll like you to help me with lecture notes on microbial physiology and metabolism and also how to go about choosing a topic for seminar presentation ( essay in microbiology)

  14. Hello! I enjoy your website. I was wondering if you could recommend a commercial machine or process for sterilizing clay used in skincare and cosmetics. I was thinking something like the dry heat sterilization process that you have listed, but I am no expert. Thank you!

  15. Hello sir, I really like your website. It’s very useful and help me to learn more. thanks for your dedication to make this website. Thank You !

  16. hello hi doctor I am master sudent from Syria can i contact with you by email, i have problem in immunity side from my research and i want to ask you if you accept, please!

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