Certain bacterial species liberate sulfur from sulfur-containing amino acids or other compounds in the form of H₂S. This ability of these bacteria can be used as an important characteristic of their identification. Hydrogen sulfide-positive organisms […]
Tinsdale agar base medium was developed by Tinsdale for the selective isolation and differentiation of C. diphtheriae from diphtheroids. Billings later modified the medium improving its differential qualities and reproducibility. Moore and Parsons used Billings’ […]
Amies transport medium is a widely used and effective semisolid medium for the transportation of swab specimens to the microbiology laboratory. Placing swabs in a moist container or transport medium prevents drying and death of […]
Culture media play a pivotal role in any microbiology laboratory for isolation, identification and sensitivity testing of different pathogens. Most of the laboratories usually prepare their own media or purchase prepared media for routine diagnostics […]
Brain Heart Infusion (BHI) broth is a general-purpose liquid medium used for the cultivation and maintenance of a wide variety of fastidious and non-fastidious microorganisms, including aerobic and anaerobic bacteria, yeast and molds from a […]
Kligler’s Iron Agar (KIA) is used for the detection of carbohydrate fermentation. Reactions of KIA helps to include/exclude particular bacterial isolate in the family Enterobacteriaceae. If an organism cannot ferment glucose, then in KIA, alkaline […]
Bacteria are often preserved in microbiology laboratory for many purposes such as to re-examine or to characterize further or to send to a national reference laboratory for confirmation etc. Selection of a particular storage method […]
Deoxycholate Citrate Agar (DCA) is a selective and differential medium, recommended for the isolation of enteric pathogens particularly Salmonella and Shigella species. Deoxycholate Citrate Agar is a modification of Deoxycholate Agar formulated by Leifson. DCA […]
Selenite broth is used as an enrichment medium for the isolation of Salmonella and Shigella from stool, urine, water and food products. It is the most widely used enrichment medium to isolate Salmonella Typhi from […]
Staib medium also known as Bird Seed Agar is a selective and differential medium for isolation of Cryptococcus neoformans from clinical specimens and differentiation of it from other Cryptococcus species. It is also known as […]
Bismuth Sulfite Agar (BS) is a selective as well as differential medium for isolation and presumptive identification of Salmonella spp especially Salmonella Typhi. Salmonella spp are the causative agent of various diseases like gastroenteritis, sepsis, enteric […]
Chromogenic Culture Media are culture medium used to isolate, identify, and differentiate specific microorganism from a heterogeneous population. The medium contains chromogenic substrate which is utilized by the microorganism to give colored colonies that is specific […]
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