Culture media are a combination of pre-defined ingredients that support the growth and proliferation of microorganisms in an artificial/in vitro environment. They are used for the isolation of organisms from various samples such as clinical specimens, food, or the environment.
Based on the consistency, culture media are further categorized as solid, semisolid, and liquid (broth). Functionally, culture media can be classified as differential, selective, enriched, enrichment, assay, transport, etc.
Phenylethyl alcohol agar (PEA) is a selective medium used to cultivate Gram-positive cocci, from a sample containing a mixed flora.
Bacterial species capable of producing H₂S release sulfide which combines with iron present in the medium forming black precipitate.
Tinsdale Agar is used for the selective isolation and differentiation of C. diphtheriae from diphtheroids.
This semisolid medium is used for the transportation of swab specimens for preserving the viability of N. gonorrhoeae, H. influenzae, N. meningitidis.
Growth supporting characteristics, physical characteristics and sterility testing of the culture media has to be tested before putting them in use.
Brain Heart Infusion (BHI) broth is a general-purpose liquid medium for the cultivation and maintenance of a wide variety of microorganisms.
Kligler Iron Agar is used for the differentiation of microorganisms on the basis of glucose and lactose fermentation and H₂S production.
Deoxycholate citrate agar is used for the selective isolation and differentiation of Salmonella and Shigella species.
Selenite broth is an enrichment medium for the isolation of Salmonella and Shigella from stool, urine, water and food products.
Bird Seed Agar is a solid medium used for selective and differential isolation of Cryptococcus neoformans from clinical specimens.
Bismuth sulfite agar is a modification of original Wilson and Blair medium used for the isolation of Salmonella typhi and other salmonellae.
It contain chromogenic substrate which is utilized by the microorganisms to give colored colonies that is specific for each microorganism.
Tryptic Soy Agar (TSA) is a general purpose culture medium for isolation and cultivation of a wide variety of organisms.
Thioglycollate broth supports the growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms.
Cary-Blair transport medium is used to transport clinical specimens suspected to contain enteric pathogens, including Shigella, Salmonella, Vibrio.
Alkaline Peptone Water (pH 8.4) is a suitable enrichment broth for the isolation of Vibrio cholerae and Vibrio species.
Salmonella-shigella (SS) agar is commonly used for the selective isolation and differentiation of Salmonella and Shigella.
Thayer-Martin agar is a selective medium used for the isolation of Neisseria gonorrheae from specimens containing a mixed flora of bacteria.
Nutrient agar is a general-purpose medium for the growth of a wide variety of non-fastidious microorganisms.
Originally formulated for dermatophyte growth and pigment production, SDA is used for growth and maintenance of fungi.
Potato Dextrose Agar (PDA) is an ideal medium for slide culture preparation. It promotes sporulation of moulds.
First reported by Baird–Parker, it is a selective medium for the enumeration of Staphylococcus aureus in foods.
Cetrimide agar test the ability of an organism to grow in the presence of cetrimide.
XLD Agar is a selective and differential medium for the isolation, cultivation, and differentiation of gram-negative enteric microorganisms.
Differential medium used for urine culture as it inhibits the swarming of Proteus and also supports the growth of gram-positive cocci.
Common transport media are Cary and Blair medium, amies medium, Staurt’s medium, and VTM for virus.
Hektoen Enteric Agar (HE) is a selective and differential medium to isolate and differentiate Salmonella and Shigella from other Enterobacteriaceae.
Fungal culture media contains high carbohydrate and nitrogen source. They may also contain cycloheximide and antibacterial agents to inhibit contaminants.
New York City medium is primarily designed for the isolation of pathogenic Neisseria. It also supports the growth of genital mycoplasmas.
TCBS agar is a selective as well as differential culture medium used for selective isolation and differentiation of Vibrio species.
Chocolate agar (heated blood agar) is an enriched medium used in clinical laboratories for cultivation of fastidious bacteria. e.g., Haemophilus influenzae.
Buffered Charcoal Yeast Extract (BCYE) Agar medium is recommended for use in the cultivation and primary isolation of Legionella spp.
Eosin methylene blue (EMB) agar is a selective and differential medium used to isolate fecal coliforms.
Blood agar is an enriched medium which supports growth of gram-positive cocci and differentiates them on the basis of hemolysis (α, β, or γ).
Selective (against gram-positive bacteria) and differential culture medium (LF and NLF), commonly used for the isolation of enterics.
Mannitol Salt Agar selects and differentiates Staphylococcus species. Staphylococcus aureus ferments mannitol and gives yellow colonies.
Best medium to use for routine susceptibility testing for non-fastidious bacteria.
TSI test determines whether a gram-negative rod utilizes glucose, and lactose or sucrose fermentatively and forms hydrogen sulfide.