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This post was most recently updated on January 15th, 2020 Real-time PCR also called quantitative PCR (qPCR) is a variant of standard polymerase chain reaction in which amplification and simultaneous quantitation of a target DNA [...] -
Nested PCR is a modification of PCR designed to increase the sensitivity and specificity of the assay reaction. It involves the use of two primer sets directed against the same target and two successive PCR [...] -
Multiplex PCR is a variant of PCR method in which more than one target sequence is amplified using multiple sets of primers within a single PCR mixture. This enables amplification of several gene segments at [...] -
Reverse Transcriptase PCR (RT-PCR) is a variation of the polymerase chain reaction that amplifies target RNA. Addition of reverse transcriptase (RT) enzyme prior to PCR makes it possible to amplify and detect RNA targets. Reverse [...] -
Blastomyces dermatitidis is a thermally dimorphic fungus that grows as a mycelial form in the environment and as a thick-walled budding yeast in tissues (at 37°C). It causes blastomycosis, a chronic granulomatous and suppurative disease [...]
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This post was most recently updated on August 26th, 2019 Streptococcus pyogenes is the leading cause of uncomplicated bacterial pharyngitis and tonsillitis commonly referred to as strep throat. Acute diseases associated with Streptococcus pyogenes occur chiefly in the respiratory tract (sinusitis, otitis), bloodstream (sepsis, [...] -
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This post was most recently updated on March 26th, 2019 Salmonella-Shigella (SS) agar is a selective and differential medium . It is used for the isolation, cultivation and differentiation of gram-negative enteric microorganisms isolated from [...] -
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The direct fluorescent antibody (DFA) test is a rapid microscopic procedure for detecting the presence of a particular antigen (typically a specific protein on the surface of a virus, bacterium or other microbes) using a [...] -
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