The direct fluorescent antibody (DFA) test is a rapid microscopic procedure for detecting the presence of a particular antigen (typically a specific protein on the surface of a virus, bacterium or other microbes) using a […]
Automated blood culture systems are the primary choice for the detection of pathogens from blood specimens. Instead of manually read broth-based conventional methods, laboratories use continuously monitored, automated blood culture systems for the processing of […]
Ames test devised by a scientist “Bruce Ames” and colleagues at the University of California in Berkeley, is a method for evaluating mutagenic effects of chemicals, drugs or implant device. This test is used to […]
Detection of specific antigens present in the sample (blood, urine, CSF or stool) using immunologic methods such as precipitin tests, particle agglutination tests, immunofluorescence, immunochromatographic or enzyme immunoassays are the mainstay for the rapid diagnosis […]
Monoclonal antibodies (mAb) are defined as the antibodies derived from a single clone of plasma cell; all having the same antigen specificity, i.e. produced against a single epitope of an antigen. Polyclonal vs Monoclonal Antibodies […]
Common antigens (or antigens of similar nature) that are possessed by a variety of phylogenetically unrelated species are known as heterophile antigens. Antibodies induced by these antigens cross-react with individual heterophilic antigen, such antibodies are […]
Pulsed-field gel electrophoresis (PFGE) is a powerful molecular typing technique by which genomic DNA is isolated from the organism of interest followed by restriction enzyme analysis. The digestion products are then analysed on an agarose […]
This post was most recently updated on October 13th, 2019Agarose gel electrophoresis is one of the most common electrophoresis technique which is relatively simple and straightforward to perform but possesses great resolving power. The agarose […]
This post was most recently updated on August 26th, 2019Culture media play a pivotal role in any microbiology laboratory for isolation, identification and sensitivity testing of different pathogens. Most of the laboratories usually prepare their […]
This post was most recently updated on August 26th, 2019Coombs test (antiglobulin test) is used to detect the presence of ‘incomplete’ Rh antibodies i.e. IgG antibodies capable of sensitising RBCs but incapable of causing agglutination […]
This post was most recently updated on August 26th, 2019Kligler’s Iron Agar (KIA) is used for the detection of carbohydrate fermentation. Reactions of KIA helps to include/exclude particular bacterial isolate in the family Enterobacteriaceae. If […]
The function of Antibody (Ab) refers to the biological effect that antibody has on a pathogen or its toxin. In addition to binding an antigen (Ag), antibodies participate in various biological activities. Though they do […]
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