Pulsed-field gel electrophoresis (PFGE) is a powerful molecular typing technique by which genomic DNA is isolated from the organism of interest followed by restriction enzyme analysis. The digestion products are then analysed on an agarose […]
Agarose gel electrophoresis is one of the most common electrophoresis technique which is relatively simple and straightforward to perform but possesses great resolving power. The agarose gel consists of microscopic pores that act as a […]
This post was most recently updated on August 26th, 2019Culture media play a pivotal role in any microbiology laboratory for isolation, identification and sensitivity testing of different pathogens. Most of the laboratories usually prepare their […]
This post was most recently updated on August 26th, 2019Coombs test (antiglobulin test) is used to detect the presence of ‘incomplete’ Rh antibodies i.e. IgG antibodies capable of sensitising RBCs but incapable of causing agglutination […]
This post was most recently updated on August 26th, 2019Kligler’s Iron Agar (KIA) is used for the detection of carbohydrate fermentation. Reactions of KIA helps to include/exclude particular bacterial isolate in the family Enterobacteriaceae. If […]
The function of Antibody (Ab) refers to the biological effect that antibody has on a pathogen or its toxin. In addition to binding an antigen (Ag), antibodies participate in various biological activities. Though they do […]
This post was most recently updated on August 26th, 2019Leptospirosis (also known as weil’s disease) is one of the widespread zoonoses caused by pathogenic spirochetes of the genus Leptospira and is found in virtually all tropical […]
T cells and B cells are white blood cells that are important cells for adaptive immunity. Like all blood cells, they are made in the bone marrow. While B-cells mature in the bone marrow, T-cells […]
This post was most recently updated on August 26th, 2019Aspergillus is a ubiquitous mold found throughout the world in air, soil, water, and decaying vegetation. It is an opportunistic pathogen causing infections mainly in people […]
This post was most recently updated on August 26th, 2019Early diagnosis of fungal infection is critical for effective treatment. For many decades, culture, direct microscopy, and histopathology have been the foundation for the diagnosis of […]
This post was most recently updated on August 26th, 2019Mycoplasma is the smallest (0.2 – 0.8 micrometers) free-living bacteria (light microscopy cannot detect it) that can pass through some filters (0.45-μm-pore-size) used to remove bacteria. […]
This post was most recently updated on August 26th, 2019Klebsiella pneumoniae is a Gram-negative rod shaped bacteria of genus Klebsiella and family Enterobacteriaceae. They are members of normal intestinal flora of humans and animals and may be isolated from […]
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