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HomeBacteriologyBiochemical tests in Microbiology

Biochemical tests in Microbiology

Hydrogen sulfide production test
Biochemical tests in Microbiology

Hydrogen Sulfide (H₂S) Production Test

March 13, 2020 Acharya Tankeshwar 1

Certain bacterial species liberate sulfur from sulfur-containing amino acids or other compounds in the form of H₂S. This ability of these bacteria can be used as an important characteristic of their identification. Hydrogen sulfide-positive organisms […]

Starch Hydrolysis Test
Bacteriology

Starch Hydrolysis Test: Principle, Procedure and Results

March 1, 2020 Nisha Rijal 0

Starch, the most important source of carbohydrate for human, is a polysaccharide mixture of two polymers, amylose and amylopectin, the latter being predominant. Amylose is a linear polysaccharide of several thousand α-D-glucose linked by 1,4-α-glycosidic […]

Furazolidone disk test
Bacteriology

Furazolidone Disk Test: Principle, Procedure and Results

December 10, 2019 Acharya Tankeshwar 0

Furazolidone (Furoxone) disk test is performed as a disk susceptibility procedure using commercially available furazolidone disks. Staphylococci are inhibited by furazolidone but micrococci and related species are resistant. This is one of the commonly used […]

Bacteriology

Kligler’s Iron Agar (KIA): Principle, Procedure and Results

April 30, 2019 Acharya Tankeshwar 4

Kligler’s Iron Agar (KIA) is used for the detection of carbohydrate fermentation. Reactions of KIA helps to include/exclude particular bacterial isolate in the family Enterobacteriaceae.  If an organism cannot ferment glucose, then in KIA, alkaline […]

Bacteriology

Carbohydrate Fermentation Test: Uses, Principle, Procedure and Results

December 10, 2016 Acharya Tankeshwar 12

The carbohydrate fermentation test is used to determine whether or not bacteria can ferment a specific carbohydrate. Carbohydrate fermentation patterns are useful in differentiating among bacterial groups or species. It tests for the presence of acid […]

Anaerobic Bacteriology

Robertson’s Cooked Meat (RCM) Medium: Principle, Composition, Procedure and Uses

November 29, 2016 Acharya Tankeshwar 2

Robertson’s Cooked Meat (RCM) medium is used for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species.   It is also known as Cooked Meat Broth (CMB) as it contains pieces of fat-free minced […]

Bacteriology

Phenylalanine Deaminase Test : Principle, Procedure, Results and Uses

November 4, 2016 Acharya Tankeshwar 2

Phenylalanine deaminase test also known as phenylpyruvic acid (PPA) test is used to test the ability of an organism to produce enzyme deaminase.  This enzyme removes the amine group from the amino acid phenylalanine and […]

Bacteriology

Elek Test: principle, procedure and results

November 23, 2015 Acharya Tankeshwar 5

Elek test is an in vitro immunoprecipitation (immunodiffusion) test to determine whether or not a strain of Corynebacterium diphtheriae is toxigenic. A test strip of filter paper containing diphtheria antitoxin is placed in the center […]

Bacteriology

Tests for Bacterial Motility: Procedure and Results

October 19, 2015 Acharya Tankeshwar 7

There are a variety of ways to determine the motility of a bacterium—biochemical tests as well as microscopic analysis. If a fresh culture of bacteria is available, microscopy is the most accurate way to determine […]

Nagler's test
Bacteriology

Nagler Reaction (Lecithinase Test): Principle, Procedure, Results, and Limitations

September 1, 2015 Nisha Rijal 3

Lecithinase test or Nagler’s reaction is a biochemical test used to identify organisms that liberate phospholipases (lecithinases) e.g. Clostridium perfringens. The alpha (α) toxin of C. perfringens has phospholipase activity and hence, helps in the […]

Biochemical tests in Microbiology

Acetate Utilization Test: Principle, Procedure, Results and Uses

May 25, 2015 Acharya Tankeshwar 1

Principle: Acetate Utilization Test is used to determine if an organism can use acetate as the sole source of carbon. (Another similar test in which organisms ability to utilize Citrate as a sole source of […]

No Picture
Biochemical tests in Microbiology

Butyrate Disk Test: Principle, Procedure, Results and Uses

May 25, 2015 Acharya Tankeshwar 0

Principle: The butyrate disk is a rapid test for the detection of the enzyme butyrate esterase in identifying Moraxella (Branhamella ) catarrhalis. This test helps to differentiate Neisseria gonorrhoeae (negative) and Moraxella catarrhalis (positive), both […]

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