This post was most recently updated on March 25th, 2020Certain bacterial species liberate sulfur from sulfur-containing amino acids or other compounds in the form of H₂S. This ability of these bacteria can be used as […]
Starch, the most important source of carbohydrate for human, is a polysaccharide mixture of two polymers, amylose and amylopectin, the latter being predominant. Amylose is a linear polysaccharide of several thousand α-D-glucose linked by 1,4-α-glycosidic […]
Furazolidone (Furoxone) disk test is performed as a disk susceptibility procedure using commercially available furazolidone disks. Staphylococci are inhibited by furazolidone but micrococci and related species are resistant. This is one of the commonly used […]
This post was most recently updated on March 25th, 2020Kligler’s Iron Agar (KIA) is used for the detection of carbohydrate fermentation. Reactions of KIA helps to include/exclude particular bacterial isolate in the family Enterobacteriaceae. If […]
This post was most recently updated on August 26th, 2019The carbohydrate fermentation test is used to determine whether or not bacteria can ferment a specific carbohydrate. Carbohydrate fermentation patterns are useful in differentiating among bacterial groups […]
This post was most recently updated on December 3rd, 2016Robertson’s Cooked Meat (RCM) medium is used for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species. It is also known as Cooked Meat […]
This post was most recently updated on April 30th, 2019Phenylalanine deaminase test also known as phenylpyruvic acid (PPA) test is used to test the ability of an organism to produce enzyme deaminase. This enzyme removes […]
This post was most recently updated on April 28th, 2016Elek test is an in vitro immunoprecipitation (immunodiffusion) test to determine whether or not a strain of Corynebacterium diphtheriae is toxigenic. A test strip of filter […]
This post was most recently updated on March 26th, 2020There are a variety of ways to determine motility of a bacterium—biochemical tests as well as microscopic analysis. If fresh culture of bacteria is available, microscopy […]
This post was most recently updated on March 26th, 2020Lecithinase test or Nagler’s reaction is a biochemical test used to identify organisms which liberate phospholipases (lecithinases) e.g. Clostridium perfringens. The alpha (α) toxin of C. […]
Principle: Acetate Utilization Test is used to determine if an organism can use acetate as the sole source of carbon. (Another similar test in which organisms ability to utilize Citrate as a sole source of […]
Principle: The butyrate disk is a rapid test for the detection of the enzyme butyrate esterase in identifying Moraxella (Branhamella ) catarrhalis. This test helps to differentiate Neisseria gonorrhoeae (negative) and Moraxella catarrhalis (positive), both […]
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