Kligler’s Iron Agar (KIA) is used for the detection of carbohydrate fermentation. Reactions of KIA helps to include/exclude particular bacterial isolate in the family Enterobacteriaceae. If an organism cannot ferment glucose, then in KIA, alkaline […]
The carbohydrate fermentation test is used to determine whether or not bacteria can ferment a specific carbohydrate. Carbohydrate fermentation patterns are useful in differentiating among bacterial groups or species. It tests for the presence of acid and/or […]
This post was most recently updated on December 3rd, 2016Robertson’s Cooked Meat (RCM) medium is used for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species. It is also known as Cooked Meat […]
This post was most recently updated on April 30th, 2019Phenylalanine deaminase test also known as phenylpyruvic acid (PPA) test is used to test the ability of an organism to produce enzyme deaminase. This enzyme removes […]
This post was most recently updated on April 28th, 2016Elek test is an in vitro immunoprecipitation (immunodiffusion) test to determine whether or not a strain of Corynebacterium diphtheriae is toxigenic. A test strip of filter […]
This post was most recently updated on May 5th, 2016There are a variety of ways to determine motility of a bacterium—biochemical tests as well as microscopic analysis. If fresh culture of bacteria is available, microscopy […]
This post was most recently updated on February 18th, 2017Lecithinase test or Nagler’s reaction is a biochemical test used to identify organisms which liberate phospholipases (lecithinases) e.g. Clostridium perfringens. The alpha (α) toxin of C. […]
Principle: Acetate Utilization Test is used to determine if an organism can use acetate as the sole source of carbon. (Another similar test in which organisms ability to utilize Citrate as a sole source of […]
Principle: The butyrate disk is a rapid test for the detection of the enzyme butyrate esterase in identifying Moraxella (Branhamella ) catarrhalis. This test helps to differentiate Neisseria gonorrhoeae (negative) and Moraxella catarrhalis (positive), both […]
This post was most recently updated on November 5th, 2016Pseudosel Agar or Cetrimide Agar is used for the selective isolation and presumptive identification of Pseudomonas aeruginosa . The medium was first developed by Lowburry and […]
This post was most recently updated on January 26th, 2016API (Analytical Profile Index) 20E presented is a biochemical panel for identification and differentiation of members of the family Enterobacteriaceae. In API 20E for identification of […]
This post was most recently updated on May 25th, 2015The nitrocefin biochemical test is a sensitive technique for detecting beta-lactamase producing strains of N. gonorrhoeae, H. influenzae, Staphylococcus spp, Enterococcus spp and Moraxella (Branhamella) catarrhalis. Nitrocefin […]
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