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Category: Biochemical Tests

link to Sulfide Indole Motility (SIM) Medium: Principle, Procedure, and Results 

Sulfide Indole Motility (SIM) Medium: Principle, Procedure, and Results 

Sulfide Indole Motility (SIM) medium is a differential bacterial growth medium for detecting three different characteristics of an organism;  Sulfur reduction and (H2S) sulfide...

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link to Casein Hydrolysis Test: Principle, Procedure, and Uses

Casein Hydrolysis Test: Principle, Procedure, and Uses

The physiological reactions of microorganisms are crucial in species identification and differentiation. As enzymes catalyze all physiological reactions, detecting the enzyme produced is an essential...

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link to Litmus Milk Test: Principle, Procedure, and Results

Litmus Milk Test: Principle, Procedure, and Results

Litmus milk is an undefined milk-based medium consisting of skim milk and the pH indicator azolitmin (litmus). Azolitmin is pink at pH 4.5 and blue at pH 8.3. Between these extremes, it is purple. In...

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link to Malonate Test: Principle, Procedure, and Results

Malonate Test: Principle, Procedure, and Results

Malonate is an ionized form of malonic acid CH2(COOH)2, and its ester and salts. The medium for the malonate test contains sodium malonate. Malonate is a reversible inhibitor of succinate...

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link to MRS Broth Test: Principle, Procedure, and Results

MRS Broth Test: Principle, Procedure, and Results

Lactobacillus MRS Broth test is used to test gas production by Gram-positive rods in a glucose-containing medium. MRS is an acronym for the authors of this publication (De Mann JD, Rogosa, and...

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link to Acetamide Utilization Test: Principle, Procedure, and Results

Acetamide Utilization Test: Principle, Procedure, and Results

Acetamide (ethanamide) is the simplest amide derived from acetic acid.  The formula of this organic compound is CH3CONH2. Acetamide utilization test is recommended for differentiating...

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