Listeria monocytogenes: Properties, Pathogenesis, and Lab Diagnosis

Listeria monocytogenes is a facultative intracellular Gram-positive coccobacilli responsible for listeriosis, one of the serious food-borne infections.

Other important distinguishing properties of this organism are as follows;

  1. It shows tumbling type of motility at 25°C but non-motile at 37°C (called differential motility, which is due to the temperature-dependent flagella expression).
  2. Unlike most other harmful bacteria, L. monocytogenes grow slowly on foods stored in a refrigerator (4°C). It is commonly found in stored foods especially aged soft cheeses, packaged meats, milk, and cold salads.
  3. Growth improves if the specimen is refrigerated at 4°C and cultured at frequent intervals, this procedure is called cold enrichment.
  4. It shows a positive CAMP test when streaked at a right angle to beta-hemolytic Staphylococcus aureus (enhanced arrowhead hemolysis).
Listeria monocytogenes in Gram staining and Culture

Transmission

Listeria monocytogenes can be found in a variety of dairy products, vegetables, fish, and meat products. Unlike most other harmful bacteria, it can grow on foods stored in a refrigerator. Consumption of food contaminated with Listeria monocytogenes is the primary mode of transmission of listeriosis. Listerosis can also be transmitted from mother to fetus if infection occurs during pregnancy.

As L. monocytogenes is able to cross the placenta, listeriosis is a major cause of perinatal and neonatal disease, typically resulting in abortion.

Pathogenesis

When ingested with contaminated foods, Listeria monocytogenes enter the epithelial cells lining gastrointestinal tracts including macrophages. It escapes from the phagosome, multiplies in the cytosol, and spreads directly to adjacent cells thus protecting itself from antibodies and complement. Various proteins (virulence factors) of this organism play role in the pathogenesis. Find more here

Diseases

Listeriosis is one of the most serious foodborne diseases. Clinical manifestation of listerosis depends on the age of the patient and other underlying conditions such as pregnancy, immunosuppression, etc.

Depending on the immune status of the person and the part of the body affected, listeriosis can cause variety of symptoms ranging from milder flu-like illness to convulsion and death.

  • Pregnant women: It affects both the mother and the fetus. Mother mostly experiences mild illness (flu-like symptoms) but it causes severe disease in the fetus or newborn baby (such as abortion, preterm delivery, newborn death, and neonatal meningitis).
  • People other than pregnant women: Fever, muscle aches, headache, stiff neck, confusion, loss of balance, and convulsions.

Most people with invasive listeriosis require hospital care, and about one in five people with the infection die. 

Laboratory Diagnosis

Sample: CSF, blood, feces or placental tissue or any other tissue specimens depending on the suspected disease and/or clinical presentation.

Direct wet mount: Listeria monocytogenes exhibits characteristic end-over-end tumbling motility when incubated in nutrient broth at room temperature for 1 to 2 hours. Presumptive identification of L. monocytogenes can be done by observation of such motility by direct wet mount.

Alternatively, suspected colonies of L. monocytogenes can be stabbed into a tube of semisolid agar and incubated at 25°C for 18 hours. Characteristics umbrella-shaped motility pattern is seen after overnight incubation.

Gram staining: Regular, short, gram-positive rods or coccobacilli occurring in pairs (resembles streptococci).

Cold enrichment

Cold enrichment is done for the isolation of Listeria monocytogenes from the placenta, tissues as well as specimens from heavily contaminated sources such as feces. The specimen is inoculated into a nutrient broth or trypticase soy broth and incubated at 4°C for one month and subcultured weekly.

Beta hemolysis of Listeria monocytogenes
Beta-hemolytic colonies of L. monocytogenes

Culture: Listeria monocytogenes can be cultured on conventional media incorporating sheep, horse or rabbit blood. Listeria monocytogenes produces round, smooth, translucent colonies with a narrow zone of beta-hemolysis. Colonies are small and give blue-green color under reflective light.

Other characteristics that helps in the identification of this bacteria are:

  • Catalase positive
  • Voges-Proskauer-positive
  • Esculin positive: L. monocytogenes is able to hydrolyze esculin in the presence of 40% bile. Esculin hydrolysis is indicated by the blackening of the medium.
  •  CAMP test  positive

Molecular diagnosis

Detection of Listeria monocytogenes DNA in CSF and tissue (fresh or paraffin blocks)

Commercial methods

  1. AccuPROBE Listeria monocytogenes Culture Identification kit: Rapid DNA probe test, which utilizes the technique of nucleic acid hybridization for the identification of Listeria monocytogenes isolated from culture.
  2. Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) method
  3. GeneQuence for Listeria monocytogenes

References and further readings

  1. Procop, G. W., & Koneman, E. W. (2016). Koneman’s Color Atlas and Textbook of Diagnostic Microbiology (Seventh, International edition). Lippincott Williams and Wilkins.
  2. Tille, P. (2017). Bailey & Scott’s Diagnostic Microbiology (14 edition). Mosby.
  3. Listeria (Listeriosis) | Listeria | CDC. (2020, March 10).
About Acharya Tankeshwar 445 Articles
Hello, thank you for visiting my blog. I am Tankeshwar Acharya. Blogging is my passion. I am working as an Asst. Professor and Microbiologist at Department of Microbiology and Immunology, Patan Academy of Health Sciences, Nepal. If you want me to write about any posts that you found confusing/difficult, please mention in the comments below.

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