Common Fungal Culture Media and their uses

Two general types of culture media are essential to ensure the primary recovery of all clinically significant fungi from clinical specimens. One medium should be non-selective (such as Brain Heart Infusion Agar; i.e., one that will permit the growth of virtually all clinically relevant fungi) and other medium should be selective, specially tailored to isolate specific pathogenic fungi of interest.

For optimal recovery of fungal pathogen, a battery of media should be used, and the followings are recommended:

  1. Media with or without cyclohexamide (Cycloheximide is added to inhibit the growth of rapidly growing contaminating molds.)
  2. Media with or without an antibacterial agent (Chloramphenicol, Gentamicin and Ciprofloxacin are commonly used antibacterial for this purpose).

Antibacterial agents are used to kill the contaminating bacterial species. If the sample is taken from sterile site, it is not necessary to use media containing antibacterial agents.

  1. Brain-heart infusion (BHI) agar: It is a non-selective fungal culture medium that permits the growth of virtually all clinically relevant fungi. It is used for the primary recovery of saprophytic and dimorphic fungi
  2. Czapek’s agar: It is used for the subculture of Aspergillus species for their differential diagnosis.
  3. Inhibitory mold agar (IMA): Primary recovery of dimorphic pathogenic fungi. Saprophytic fungi and dermatophytes will not be recovered.
  4. Mycosel/Mycobiotic agar:
    1. It is generally Sabouraud’s dextrose agar with cycloheximide and chloramphenicol added.
    2. It is used for the primary recovery of dermatophytes.
    3. Niger Seed Agar: It is used for the identification of Cryptococcus neoformans.
  5. Potato Dextrose Agar (PDA): It is a relatively rich medium for growing a wide range of fungi.
  6. Sabouraud’s Heart Infusion (SABHI) agar: Primary recovery of saprophytic and dimorphic fungi, particularly fastidious strains.

    Penicillium notatum on Sabouraud agar Image source: ASM
    Penicillium notatum on Sabouraud agar
    Image source: ASM
  7. Sabouraud’s dextrose agar (SDA):
    1. Sabouraud’s agar is sufficient for the recovery of dermatophytes from cutaneous samples and yeasts from vaginal cultures.
    2. Not recommended as a primary isolation medium because it is insufficiently rich to recover certain fastidious pathogenic species, particularly most of the dimorphic fungi.
    3.  Sabouraud’s dextrose agar (2%) is most useful as a medium for the subculture of fungi recovered on enriched medium to enhance typical sporulation and provide the more characteristic colony morphology.
  8. Potato flake agar: Primary recovery of saprophytic and dimorphic fungi, particularly fastidious and slow growing strains.
Further reading and sources: For more information please read the books:
  1. Koneman’s color atlas and textbook of diagnostic microbiology and
  2. Bailey and Scott’s Diagnostic Microbiology. 

5 thoughts on “Common Fungal Culture Media and their uses

  1. i beleve potato flake agar and potato dextrin agar are fundementaly the same, however PDA is prepared fresh from potatoes rather than dried and reconstituted.

    “This communication
    describes the formulation of a medium which is
    equivalent to home-made potato dextrose agar
    in the promotion of conidiation by molds…
    The use of commercially available potato
    flakes resulted in this formulation being named
    potato flakes agar (PFA).”

    1. Tankeshwar Acharya

      - Edit


      Dear Seb
      Thank you so much for your valuable information. Your feedback is very important for me, so that microbeonline can provide accurate information. There might have some lapses, if any, i will try to correct these. I have read both of your comments, some agar that i have missed, might be because such agar is not popular in my country, but i follow your suggestions.

  2. i apologise for the double post however I also noticed that you haven’t included malt extract agar which is certainly the most important for yeast culture. also, as antibiotics (and other additives to create a more selective media) can generally be added to any base media, I feel it is misleading to repeat agars with these.
    your choice to include common combinations of other agars on your list is confusing but presumably as their name can be used to imply other ingredients balanced for the combination’s application.

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