Two general types of culture media are essential to ensure the primary recovery of all clinically significant fungi from clinical specimens. One medium should be non-selective (such as brain heart infusion agar; i.e., one that will permit the growth of virtually all clinically relevant fungi) and other media should be selective, specially tailored to isolate specific pathogenic fungi of interest.
For optimal recovery of the fungal pathogen, a battery of media should be used, and the followings are recommended:
- Media with or without cycloheximide (cycloheximide is added to inhibit the growth of rapidly growing contaminating molds.)
- Media with or without an antibacterial agent (chloramphenicol, gentamicin, and ciprofloxacin are commonly used antibacterial for this purpose).
Antibacterial agents are used to killing the contaminating bacterial species. If the sample is taken from a sterile site, it is not necessary to use media containing antibacterial agents.
Table of Contents
Common Fungal Culture Media
| Fungal Culture Media | Essential ingredients | Intended use |
| Birdseed agar | Guizotia abyssinica seeds, glucose, creatinine, chloramphenicol, monopotassium phosphate, agar | Selective and differential isolation of Cryptococcus neoformans from clinical specimens. |
| Brain-heart infusion agar | Brain heart infusion, glucose, L-cysteine hydrochloride, agar | Growth of fastidious pathogenic fungi such as Histoplasma capsulatum and Blastomyces dermatitidis. |
| CHROMagar Candida medium | Peptone, glucose, chloramphenicol, ‘Chromogenic ix’, agar | Selective and differential chromogenic medium for the isolation and identification of various Candida species |
| Cornmeal agar/ Cornmeal tween agar | Cornmeal, Tween 80, agar | Identification of C. albicans by microscopic morphology or chlamydospore production. |
| Czapek-Dox agar | NaNO3, K2HO4, KCl, MgSO4, FeSO4, glucose, agar | Identification of Aspergillus and Penicillium species. It can also be used for chlamydospore production by Candida albicans. |
| Dermatophyte test medium (DTM) | Soy peptone, dextrose, cycloheximide, chloramphenicol, gentamicin sulfate, phenol red, agar | Primary and differential fungal cultures medium to isolate and identify dermatophytes. |
| Inhibitory mold agar (IMA) | Tryptone, beef extract, yeast extract, starch, dextrin, chloramphenicol, gentamicin, and saline buffer | Primary recovery of pathogenic fungi exclusive of dermatophytes. |
| Mycosel/mycobiotic agar | Papaic digest of soybean meal, dextrose, cycloheximide, chloramphenicol, agar | Highly selective medium; recommended for the isolation of pathogenic fungi from materials containing a large amount of fungal and bacterial flora. |
| Potato dextrose agar (PDA) | Potato extract, potato infusion, dextrose, agar | Ideal medium for slide culture preparation. It promotes sporulation of dermatophytes. Demonstration of pigment production by T. rubrum. |
| Potato flake agar | Potato flakes, dextrose, agar | Primary recovery of saprophytic and pathogenic fungi. |
| Rice starch agar | Cream of rice, Tween 80, agar | Production of chlamydospore in Candida albicans |
| Sabouraud dextrose agar (SDA) | Pancreatic digest of casein, glucose, agar | Growth and maintenance of clinically important fungal pathogens. |
Brain-heart infusion (BHI) agar
It is a non-selective fungal culture medium that permits the growth of virtually all clinically relevant fungi. It is used for the primary recovery of saprophytic and dimorphic fungi.
Czapek dox agar
Czapek dox agar is a semisynthetic medium containing sodium nitrate as the sole source of nitrogen. It is used for the subculture of Aspergillus species for their differential diagnosis.
Inhibitory mold agar (IMA)
Inhibitory mold agar (IMA) is an enriched medium containing chloramphenicol (with some formulations containing gentamicin). It supports the growth of a wide range of fungi and may inhibit bacterial growth due to its antibiotic content. It is used for the primary recovery of dimorphic pathogenic fungi. Saprophytic fungi and dermatophytes will not be recovered.
Mycosel/Mycobiotic agar
- It is generally Sabouraud’s dextrose agar with cycloheximide and chloramphenicol added.
- It is used for the primary recovery of dermatophytes.
Niger Seed Agar
It is used for the identification of Cryptococcus neoformans.
Potato Dextrose Agar (PDA)
Potato Dextrose Agar (PDA) is a common fungal growth media made from an infusion of potato and dextrose. It can be supplemented with acid or antibiotics to inhibit bacterial growth. It is recommended for plate count methods for microbial testings of foods, dairy products, and cosmetics.
Sabouraud’s Heart Infusion (SABHI) agar
Primary recovery of saprophytic and dimorphic fungi, particularly fastidious strains.
Image source: ASM
Sabouraud’s dextrose agar (SDA)
- Sabouraud’s agar is sufficient for the recovery of dermatophytes from cutaneous samples and yeasts from genital cultures.
- Not recommended as a primary isolation medium because it is insufficiently rich to recover certain fastidious pathogenic species, particularly most of the dimorphic fungi.
- Sabouraud’s dextrose agar (2%) is most useful as a medium for the subculture of fungi recovered on enriched medium to enhance typical sporulation and provide the more characteristic colony morphology.
Potato flake agar
Primary recovery of saprophytic and dimorphic fungi, particularly fastidious and slow-growing strains.
Further reading and sources
i beleve potato flake agar and potato dextrin agar are fundementaly the same, however PDA is prepared fresh from potatoes rather than dried and reconstituted.
“This communication
describes the formulation of a medium which is
equivalent to home-made potato dextrose agar
in the promotion of conidiation by molds…
The use of commercially available potato
flakes resulted in this formulation being named
potato flakes agar (PFA).”
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC272272/pdf/jcm00155-0213.pdf
Dear Seb
Thank you so much for your valuable information. Your feedback is very important for me, so that microbeonline can provide accurate information. There might have some lapses, if any, i will try to correct these. I have read both of your comments, some agar that i have missed, might be because such agar is not popular in my country, but i follow your suggestions.
i apologise for the double post however I also noticed that you haven’t included malt extract agar which is certainly the most important for yeast culture. also, as antibiotics (and other additives to create a more selective media) can generally be added to any base media, I feel it is misleading to repeat agars with these.
your choice to include common combinations of other agars on your list is confusing but presumably as their name can be used to imply other ingredients balanced for the combination’s application.
hello sir i wants to know that for growing of T.mentagrophytes ,M.canis,which medium we can used other than Sabouraud’s Dextrose agar medium.
PFA-potato flake agar with chloramphenicol and gentamicin is good for the above said molds
How to find micro organisms in honey??? And which media is involved???
Sir g esse kuch bhi related material ho please sand kr do
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I’m working on synthesizing Molybdenum nanoparticles using Aspergillus strains. How good is the Rose bengal Agar media for selective culturing of Aspergillus?
I am using SDA for Total Y&M testing,if there is 2 creamy-white colonies growth on SDA plate, can I report the result as Y&M=2 cfu/g? or that is be only Yeast?
Where can i find fu agar??