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Category: Staining Techniques

link to Acridine Orange Staining: Principle, Procedure, Results

Acridine Orange Staining: Principle, Procedure, Results

Acridine orange is a fluorescent dye that intercalates or binds with the nucleic acid (either DNA or RNA) present in organisms and fluoresces to emit various colors that help differentiate cellular...

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link to Albert Stain: Principle, Procedure and Results

Albert Stain: Principle, Procedure and Results

Albert stain is a type of differential stain used for staining high-molecular-weight polymers of polyphosphate known as metachromatic granules or volutin granules found in Corynebacterium...

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link to Endospore Staining: Principle, Procedure, Results

Endospore Staining: Principle, Procedure, Results

Endospore staining is a differential technique that selectively stains the spores and makes them distinguishable from the vegetative part of the cells. Endospores are produced by a few genera of...

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link to Auramine-Rhodamine Fluorochrome Staining

Auramine-Rhodamine Fluorochrome Staining

Auramine-rhodamine fluorochrome staining, also known as "Truant method of staining," is used to visualize acid-fast bacilli (AFB). Ziehl-Neelsen (hot) and Kinyoun (cold) are still widely used in...

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link to Gram Staining: Principle, Procedure, Results

Gram Staining: Principle, Procedure, Results

Danish physician Hans Christian Gram developed the Gram staining method in 1884. Gram staining procedure uses four chemicals; crystal violet, iodine, alcohol, and safranin, to stain...

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link to Lactophenol Cotton Blue (LPCB) Mounts

Lactophenol Cotton Blue (LPCB) Mounts

The lactophenol cotton blue (LPCB) wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare. The preparation has the following...

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