Auramine-rhodamine fluorochrome staining also known as "Truant method of staining", is used to visualize acid-fast bacilli (AFB). Ziehl-Neelsen (hot), Kinyoun (cold) are still widely used methods in...
Category: Staining Techniques
Danish physician Hans Christian Gram developed the Gram staining method in 1884. To stain bacteria, it uses four chemicals, crystal violet, iodine, alcohol, and safranin. Gram staining is...
The lactophenol cotton blue (LPCB) wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare. The preparation has the following...
Bacteria are easier to detect in broth culture if the preparation is stained using toluidine blue 0.5% w/v, rather than stained by the Gram staining technique. Procedure of Toluidine Blue Staining...
Most motile bacteria possess flagella, the shape, number, and position of which are important characteristics in the differentiation of genera and species identification. Staining bacterial...
Ziehl-Neelsen (ZN) acid-fast staining technique is used to stain Mycobacterium species, including M. tuberculosis, M. ulcerans, M. leprae, and nontuberculous mycobacteria (NTM). Detection of...
