Bacteria are easier to detect in broth culture if the preparation is stained using toluidine blue 0.5% w/v, rather than stained by the Gram staining technique. Procedure of Toluidine Blue Staining...
Category: Staining Techniques
Most motile bacteria possess flagella. The shape, number, and position of flagella are important characteristics in differentiating genera and species identification. Staining bacterial...
Ziehl-Neelsen (ZN) acid-fast staining technique is used to stain Mycobacterium species, including M. tuberculosis, M. ulcerans, M. leprae, and nontuberculous mycobacteria (NTM). Detection of...
Structural details of organisms cannot be seen under a light microscope due to a lack of contrast. Hence, we use dyes to stain cells. Dyes bind with cellular constituents producing color contrast and...
