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Homegram negative rods

gram negative rods

Bacteriology

Cary-Blair transport medium: Composition, Preparation and Uses

September 10, 2016 Acharya Tankeshwar 3

Cary-Blair transport medium can be used to transport of clinical specimens suspected to contain enteric pathogens, including  Shigella, Salmonella, Vibrio cholerae, and Escherichia coli O157:H7. Since this transport medium has a high pH (8.4), viability […]

Bacteriology

Salmonella-Shigella (SS) Agar:  Composition, Principle, Procedure and Results

September 6, 2016 Acharya Tankeshwar 7

Salmonella-Shigella (SS) agar is a selective and differential medium . It is used for the isolation, cultivation and differentiation of gram-negative enteric microorganisms isolated from both clinical and non-clinical specimens such as from feces, urine, and […]

Bacteriology

Voges Proskauer (VP) Test: Principle, Procedure and Results

March 17, 2015 Acharya Tankeshwar 8

Voges-Proskauer is a double eponym, named after two microbiologists working at the beginning of the 20th century.  They first observed the red color reaction produced by appropriate culture media after treatment with potassium hydroxide. It […]

Bacteriology

Culture media used for isolation and identification of Salmonella typhi and paratyphi

January 27, 2015 Acharya Tankeshwar 11

Salmonella is a member of the family Enterobacteriaceae. It is a Gram-negative bacilli, motile and non-lactose fermenter. The genus can be divided into two species (S. enterica and S. bongori), based on their phenotypic profile. […]

Bacteriology

Vibrio Cholerae laboratory diagnosis and confirmation

October 23, 2013 Acharya Tankeshwar 1

Sample: Fresh diarrheal stool or stool collected in Cary Blair transport medium. Macroscopic examination: Rice watery stool with mucus flecks  The laboratory diagnosis of cholera is based on colony morphology, culture characteristics, biochemical reactions, and […]

Bacteriology

Enterobacteriaceae Family: Common Characteristics

October 1, 2013 Acharya Tankeshwar 10

Enterobacteriaceae family contains a large number of genera that are biochemically and genetically related to one another. Many of the traditional or familiar bacteria are found in this family e.g. Escherichia, Shigella, Salmonella, Enterobacter, Proteus, […]

Bacteriology

Differences between Gram positive and Gram Negative bacteria

August 11, 2013 Acharya Tankeshwar 6

The cell wall of Gram negative bacteria is more complex than those of Gram positive bacteria. Gram negative bacteria contain an extra layer of cells called outer membrane or LPS layer which surrounds the thin […]

IMViC Test results of Escherichia coli
Bacteriology

IMViC Tests: Principle, Procedure and results

July 21, 2013 Acharya Tankeshwar 23

Each of the letters in “IMViC” stands for one of these tests. “I” is for indole; “M” is for methyl red; “V” is for Voges-Proskauer, and “C” is for citrate,  lowercase “i” is added for […]

Bacteriology

Campylobacter Jejuni: Disease, Properties and Laboratory diagnosis

May 18, 2013 Acharya Tankeshwar 1

Campylobacter usually causes diarrhea (often bloody). It is the most common cause of gastroenteritis worldwide, enterocolitis, especially in children. It causes systemic infection (rarely) in children and debilitated adults. Systemic infections (bacteremia), mostly by Campylobacter intestinalis. […]

Bacteriology

Lipopolysaccharide (LPS) of Gram Negative Bacteria, characteristics and functions

April 29, 2013 Acharya Tankeshwar 5

Lipopolysaccharide (LPS) layer also called the outer membrane is the outer most layer  present in the cell wall of gram negative bacteria.  It is a characteristics feature of Gram negative bacteria.  As in peptidoglycan biosynthesis, […]

Bacteriology

Salmonella: Properties, Disease and Laboratory diagnosis

April 27, 2013 Acharya Tankeshwar 1

Salmonella is a Gram-negative, rod-shaped, motile bacilli which move with the use of its peritrichous flagella. The genus Salmonella can be divided into two species (S. enterica and S. bongori), based on their phenotypic profile. […]

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