Streptococcus agalactiae (GBS): Properties, Pathogenesis and Laboratory Diagnosis

Streptococcus agalactiae, also known as Group B Streptococcus (GBS), is a major cause of neonatal sepsis and meningitis. Other infections caused by Group B Streptococci include postpartum endometritis and bacteremia among pregnant women and pneumonia, endocarditis, skin and soft tissue infections, etc., in adults with underlying diseases.

Properties of Streptococcus agalactiae

General Properties

  1. Gram-positive cocci in pairs and chains
  2. Capsulated: S.agalactiae has 10 distinct serotypes including nine capsular serotypes; Ia, Ib, and II to VIII  and one nontypeable strain (serotype IX)
  3. Facultative anaerobic
  4. Non-sporing

Pathogenesis

Virulence Factors of Streptococcus agalactiae

  1. Polysaccharide capsular antigens: Inhibits activation of the alternative complement cascade and prevents phagocytosis
  2. C5a peptidase: C5a peptidase produced by the streptococci cleave complement component C5a and interfere with C5a-mediated neutrophil chemotaxis. This peptidase also binds to fibronectin and serves as a bacterial adhesin and invasin
  3. Beta-hemolysin/cytolysin: Pore-forming hemolysin that is able to lyse pulmonary alveolar epithelial and endothelial cells and plays a role in neonatal lung infection and subsequent sepsis.
  4. Lipotechoic acid: Participate in facilitating adherence as the first step in infection.
  5. Cell surface proteins:
    1. C antigen:
      1. May mediate internalization of organisms within human cervical epithelial cells following attachment.
      2. Protect organisms from intracellular killing following phagocytosis
    2. Cell surface penicillin-binding protein (PBP 1a): Enables streptococcal cells to resist intracellular killing by phagocytic cells.
    3. Hyaluronic acid lyase: Which may act to spread infection by the breakdown of hyaluronic acid in the extracellular matrix, and may act on the hyaluronic acid present in concentrations in placental tissues, fetal tissues, and amniotic fluids.

Disease & Clinical Presentation of Streptococcus agalactiae

Rectovaginal colonization of Streptococcus agalactiae is found in 10% to 37% of pregnant women which may be transient, intermittent, or persistent. The presence of group B streptococci in the female genital tract at the time of birth can lead to infection of the neonate.

Neonatal disease with group B streptococci follows two patterns, termed early-onset disease and late-onset disease.

  1. Early-onset disease:
    1. is associated with in utero or perinatal (during passage through a birth canal that is colonized with group B streptococci) organism acquisition.
    2. Onset of disease occurs within 12 hours to 5 days after birth.
    3. The disease spectrum includes bacteremia, pneumonia, meningitis, septic shock, and neutropenia.
  2. Late-onset-disease:
    1. acquired from the birth canal of colonized mothers or from postnatal acquisition from the mother, other caregivers, or nosocomially
    2. Onset of disease occurs within 7 days to 3 months (average 3 to 4 weeks) after birth.
    3. Bacteremia is the predominant clinical presentation, and about 25% of these infants also develop group B streptococcal meningitis.

Laboratory diagnosis

Specimen Collection

  • Detection of carriers (i.e. perinatal detection of GBS): One vaginal and one rectal swab, collected and sent in Amies of Stuart’s medium without charcoal, within 4 hours of sample collection.
  • Diagnosis of cases: CSF, blood and other samples (depending on clinical presentations).

Enrichment broth: Todd-Hewitt broth with antimicrobials (gentamicin, nalidixic acid, or colistin) is used to suppress the growth of vaginal flora and allow the growth of S. agalactiae.

The presence of Gram-positive cocci in short chains in the CSF of neonates is presumptively identified as Streptococcus agalactiae.

A: Culture

Culture media: Blood Agar

Beta-hemolytic colonies of Streptococcus agalactiae
Beta-hemolytic colonies of S. agalactiae
(Image source: wikimedia.org)

Isolation of Streptococcus agalactiae from a genital site of a woman at term should be communicated as an urgent report.

Colony characteristics in blood agar: Colonies are larger than group A streptococci; translucent to opaque; flat, glossy; narrow zone of beta-hemolysis. Some strains are non-hemolytic.

Test for the identification of suspected GBS (S. agalactiae) colonies

  • Catalase test: When we observe beta-hemoytic colonies in blood agar, performing catalase test, helps to differentiate between beta-hemolytic streptococci from staphylococci. Staphylococci are catalase-positive whereas streptococci are catalase-negative.
CAMP Test
CAMP test : A. Streptococcus agalactiae (positive)
B. Streptococcus pyogenes (negative)
Image source: ASM
  • CAMP test: CAMP test may be employed for presumptive identification of catalase-negative, beta-hemolytic streptococci. S. agalactiae gives enhanced arrowhead beta-hemolysis at the juncture of S.agalactiae and S. aureus streaks; when streaked at perpendicular with beta-hemolytic S. aureus in blood agar.

B: Other tests

  • Latex agglutination: Several commercial antigen detection kits are available for diagnosis of neonatal sepsis and meningitis using serum CSF or urine as a sample.
  • DNA probe, nucleic acid amplification test (NAAT), or other molecular testing are also available for the identification of S. agalactiae.

References and further readings

About Acharya Tankeshwar 455 Articles
Hello, thank you for visiting my blog. I am Tankeshwar Acharya. Blogging is my passion. I am working as an Asst. Professor and Microbiologist at Department of Microbiology and Immunology, Patan Academy of Health Sciences, Nepal. If you want me to write about any posts that you found confusing/difficult, please mention in the comments below.

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