Southern blotting is a molecular technique to find target DNA sequences in a sample. It is a multi-step process, that begins with the electrophoresis of DNA, transfer of DNA fragments into...
Category: Molecular Biology
Real-time PCR also called quantitative PCR (qPCR), is a variant of standard polymerase chain reaction in which amplification and simultaneous quantitation of a target DNA is done in the same PCR...
Nested PCR is a modification of PCR designed to increase the sensitivity and specificity of the assay reaction. It involves the use of two primer sets directed against the same target and two...
Multiplex PCR is a variant of PCR methods in which more than one target sequence are amplified using multiple sets of primers within a single PCR mixture. This enables amplification of several gene...
Reverse Transcriptase PCR (RT-PCR) is a variation of the polymerase chain reaction that amplifies target RNA. Addition of reverse transcriptase (RT) enzyme prior to PCR makes it possible to amplify...
The lac operon is a well-known example of an inducible gene network that regulates the transport and metabolism of lactose in Escherichia coli. It encodes the genes for the internalization of...
