Real-time PCR also called quantitative PCR (qPCR), is a variant of standard polymerase chain reaction in which amplification and simultaneous quantitation of a target DNA is done in the same PCR...
Category: Molecular Biology
Nested PCR is a modification of PCR designed to increase the sensitivity and specificity of the assay reaction. It involves the use of two primer sets directed against the same target and two...
Multiplex PCR is a variant of PCR methods in which more than one target sequence are amplified using multiple sets of primers within a single PCR mixture. This enables amplification of several gene...
Reverse Transcriptase PCR (RT-PCR) is a variation of the polymerase chain reaction that amplifies target RNA. Addition of reverse transcriptase (RT) enzyme prior to PCR makes it possible to amplify...
Pulsed-Field Gel Electrophoresis (PFGE): Steps, Applications
Pulsed-field gel electrophoresis (PFGE) is a powerful molecular typing technique by which genomic DNA is isolated from the organism of interest, followed by restriction enzyme analysis. The digestion...
Agarose gel electrophoresis is one of the most common electrophoresis techniques which is relatively simple and straightforward to perform but possesses great resolving power. The agarose gel...
