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Category: Molecular Biology

link to Southern Blotting: Principle, Steps, Applications

Southern Blotting: Principle, Steps, Applications

Southern blotting is a molecular technique to find target DNA sequences in a sample. It is a multi-step process, that begins with the electrophoresis of DNA, transfer of DNA fragments into...

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link to Real-time PCR: Principles and Applications

Real-time PCR: Principles and Applications

Real-time PCR also called quantitative PCR (qPCR), is a variant of standard polymerase chain reaction in which amplification and simultaneous quantitation of a target DNA is done in the same PCR...

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link to Nested PCR: Principle and Applications

Nested PCR: Principle and Applications

Nested PCR is a modification of PCR designed to increase the sensitivity and specificity of the assay reaction. It involves the use of two primer sets directed against the same target and two...

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link to Multiplex PCR: Principle, Applications

Multiplex PCR: Principle, Applications

Multiplex PCR is a variant of PCR methods in which more than one target sequence are amplified using multiple sets of primers within a single PCR mixture. This enables amplification of several gene...

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link to Reverse transcriptase (RT)-PCR: Principles, Applications

Reverse transcriptase (RT)-PCR: Principles, Applications

Reverse Transcriptase PCR (RT-PCR) is a variation of the polymerase chain reaction that amplifies target RNA. Addition of reverse transcriptase (RT) enzyme prior to PCR makes it possible to amplify...

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link to Pulsed-Field Gel Electrophoresis (PFGE): Steps, Applications

Pulsed-Field Gel Electrophoresis (PFGE): Steps, Applications

Pulsed-field gel electrophoresis (PFGE) is a powerful molecular typing technique by which genomic DNA is isolated from the organism of interest, followed by restriction enzyme analysis. The digestion...

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