This post was most recently updated on May 13th, 2017McFarland turbidity standards are prepared by mixing various volumes of 1% sulfuric acid and 1% barium chloride to obtain solutions with specific optical densities. 0.5 McFarland […]
This post was most recently updated on May 25th, 2015The nitrocefin biochemical test is a sensitive technique for detecting beta-lactamase producing strains of N. gonorrhoeae, H. influenzae, Staphylococcus spp, Enterococcus spp and Moraxella (Branhamella) catarrhalis. Nitrocefin […]
This post was most recently updated on May 13th, 2017Epsilometer test (E- test ) is an ‘exponential gradient’ method of determination of antimicrobial resistance. The E-test has been developed to provide a direct quantification of antimicrobial […]
This post was most recently updated on May 13th, 2017Modified Hodge Test (MHT) is a simple phenotypic test for detection of presence of carbapenemase enzyme in bacteria. Positive MHT test is observed in Klebsiella pneumoniae […]
This post was most recently updated on November 26th, 2019Stokes Disc Diffusion Technique varies from Kirby Bauer disc diffusion in the use of both control and test strain on a same plate. Stokes disc diffusion technique […]
This post was most recently updated on November 26th, 2019Modified Kirby-Bauer disc diffusion test method is a reference method which could be used as a routine technique to test the sensitivity of the isolate in […]
This post was most recently updated on June 9th, 2016World Health organization has published the basic set of drugs for routine susceptibility testing in various situations. The drugs are divided in to two sets. Set […]
This post was most recently updated on May 10th, 2017When the result of antimicrobial susceptibility testing is reported to the clinician, pathogenic microorganisms are classified into one of the three categories; “Resistant”, “Intermediate” or “Susceptible”. Other […]
This post was most recently updated on October 28th, 2016Antimicrobial agents that are chosen for testing against a particular bacterial isolate are referred to as the antimicrobial battery or panel. Selecting the appropriate antimicrobials for susceptibility […]
This post was most recently updated on May 13th, 2017Preparation of antibiotic stock solutions Antibitiotics may be received as powders or tablets. It is recommended to obtain pure antibiotics from commercial sources, and not use […]
This post was most recently updated on August 30th, 2013Inducible clindamycin resistance in staphylococci can be detected by disk diffusion method using Clindamycin and Erythromycin disks. D test is performed by disk diffusion, placing a […]
This post was most recently updated on September 9th, 2019Mueller-Hinton agar (MHA) is the best medium to use for routine antimicrobial susceptibility testing using Kirby-Bauer disc diffusion method for nonfastidious bacteria (both aerobe and facultative anaerobe). Use of […]
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