Antimicrobial Susceptibility Testing (AST): Guidelines and Best Practices
Antibiotic susceptibility testing should be performed only on clinically significant isolates, not on all microorganisms recovered in culture. Clinical and Laboratory Standards Institute (CLSI)...
Antimicrobial susceptibility testing is most often used for guidance in the therapy of bacterial infections. When the result of antimicrobial susceptibility testing is reported to the clinician,...
Preparation of antibiotic stock solutions Antibiotics may be received as powders or tablets. It is recommended to obtain pure antibiotics from commercial sources, and not use injectable...
Cartridges containing commercially prepared antimicrobial discs for susceptibility testing are generally packaged to ensure appropriate anhydrous conditions. Temperature and moisture are the...
Inducible clindamycin resistance in staphylococci and streptococci can be detected by the disk diffusion method using clindamycin and erythromycin disks or broth microdilution methods. D-zone test is...
Mueller-Hinton agar (MHA) is the best medium for routine antimicrobial susceptibility testing using the Kirby-Bauer disc diffusion method for non-fastidious bacteria (aerobe and facultative...
