Microbiology Laboratory Safety Rules and Procedure

By Acharya Tankeshwar •  Updated: 05/28/22 •  3 min read

Safety in a microbiology laboratory is important in the prevention of infection because virulent and/or potentially pathogenic microorganisms are cultured (grown) there.  In addition to microorganisms, there are some chemicals used in this laboratory that are potentially harmful. Many procedures involve glassware, open flames, and sharp objects that can cause trauma/ damage if used improperly.

You will find some of these rules and procedures listed in the beginning material of your laboratory manual. If you find any safety rules or procedures listed here appear to be in conflict with those given in your laboratory manual, please resolve it by asking your course instructor or teaching assistant (TA) for clarification.

General Safety Rules and Procedures

No smoking eating or drinking

The laboratory procedures must be read prior to attending the laboratory session.

  1. Smoking, eating, and drinking in the laboratory are absolutely prohibited in the laboratory at any time.

2. Only closed-toe shoes are to be worn in the laboratory. Sandals or open-toed or canvas shoes are not permitted because of the constant danger of cuts and infections from broken glass found on the lab floors and the possibility of chemical spills.

laboratory safety symbol

3. Keep hands and other objects away from your face, nose, eyes, ears, and mouth. The application of cosmetics in the laboratory is prohibited in the laboratory

4. Work areas/surfaces must be disinfected before and after use.

Wear Lab Coats

5. Laboratory coats must be worn and buttoned while in the laboratory. Laboratory coats should not be worn outside the laboratory.

6. Protective eyewear must be worn when performing any exercise or procedure in the laboratory.

7. Long hair should be secured behind your head to minimize fire hazards or contamination of experiments.

8. Hands must be washed before leaving the laboratory.

9. Upon entering the laboratory, coats, books, and other paraphernalia e.g. purses, briefcases, etc should be placed in specified locations and never on benchtops (except for your lab manual).

No mouth pipetting

10. Never pipette anything by mouth (including water). Always use pipetting devices.

11. Dispose of wastes in their proper containers (see Biohazard Waste Disposal below).

12. When handling chemicals, note the hazard code on the bottle and take the appropriate precautions indicated.

13. Do not pour chemicals down the sink.

14. Return all chemicals, reagents, cultures, and glassware to their appropriate places.

15. Do not pour biohazardous fluids down the sink.

16. Glassware should be washed with soap and water, then rinsed with distilled water.

17. Flame transfer loops, wires, or needles before and immediately after use to transfer biological material.

18. Do not walk around the laboratory with transfer loops, wires, needles, or pipettes containing infectious material.

19. Be careful around Bunsen burners. Flames cannot always be seen.

20.Turn off incinerators before leaving the laboratory.

21. Report any broken laboratory equipment, immediately, report any broken glass, especially those containing infectious materials.

22. If you are injured in the laboratory, immediately contact your course instructor or TA. Spills, cuts and other accidents should be reported to the instructor or TA in case further treatment is necessary.

23. Familiarize yourself with safety equipment in the laboratory and emergency escape routes.

24. Always wipe and clean the lenses of your microscope before putting them away. Use the appropriate tissue paper and cleaning solution for this purpose.

25. Use appropriate universal precautions with all biological fluids.

26. Do not remove any materials from the laboratory without the written permission of the course instructor or TA.

Acharya Tankeshwar

Hello, thank you for visiting my blog. I am Tankeshwar Acharya. Blogging is my passion. As an asst. professor, I am teaching microbiology and immunology to medical and nursing students at PAHS, Nepal. I have been working as a microbiologist at Patan hospital for more than 10 years.

4 responses to “Microbiology Laboratory Safety Rules and Procedure”

  1. Lina says:

    Hello, I am a MLS student and I have my bacteriology rotation this summer. I have around two weeks before my rotation starts and I am considering a rhinoplasty at the beginning of my break. Can i do my surgery and enter the lab?

    • Tankeshwar Acharya says:

      Dear Lina,
      Wishing you all the best for your Rhinoplasty. You know that, Microbiology laboratory is in fact a dangerous place as it grows and manipulates various pathogens so we have to follow standard safety protocols depending on the nature of laboratory and the organisms being cultured/manipulated there. Its always a good idea to consult with your Physician as well as professor.

  2. Oladosu ABIOLA says:

    Pls Sir help me to solve this problem, there is a topic before me wish say (safety technique in bacteriology) is a seminer presentation

  3. Muhammad Sabir says:

    Sir I am very thankful to you…..
    Your available material is very helpful for me.
    Allah help you in every aspect of life.

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