Enterobacteriaceae

API and RAPID ID For Microbial Identification

API and RAPID ID For Microbial Identification

BioMérieux's API identification products are test kits for the identification of Gram-positive bacteria, Gram-negative bacteria, and yeast.

Serratia marcescens: Properties, Pathogenesis, Diagnosis

Serratia marcescens: Properties, Pathogenesis, Diagnosis

Enteric gram-negative rod, produces red-pigmented colonies. It causes hospital-acquired pneumonia, UTI and sepsis.

Yersinia pestis: Properties, Disease, Lab Diagnosis

Yersinia pestis: Properties, Disease, Lab Diagnosis

Yersinia pestis is a short gram-negative rod, responsible for deadly zoonotic disease, plague.

Klebsiella pneumoniae: Properties, Diseases, Lab Diagnosis

Klebsiella pneumoniae: Properties, Diseases, Lab Diagnosis

This gram-negative capsulated rod-shaped bacteria causes hospital acquired pnuemonia, UTI and blood stream infection.

Proteus species: Properties, Diseases, Identification

Proteus species: Properties, Diseases, Identification

Known for its swarming motility, Proteus is a gram negative rod of Enterobacteriaceae family and causing urinary tract infections.

Methyl Red (MR) Test: Principle, Procedure, Results

Methyl Red (MR) Test: Principle, Procedure, Results

Certain bacteria ferment glucose to produce stronger acids that take the media pH below 4.4 in which Methyl Red indicator turns red.

Catalase test: Principle, Procedure, Results, Uses

Catalase test: Principle, Procedure, Results, Uses

Catalase test is used to distinguish among Gram-positive cocci. Staphylococci are catalase-positive but Streptococci are catalase-negative.

Enterobacteriaceae Family: Common Characteristics

Enterobacteriaceae Family: Common Characteristics

Enterobacteriaceae is a family of gram-negative rods which are catalase positive and oxidase negative.

IMViC Tests: Principle, Procedure, Results

IMViC Tests: Principle, Procedure, Results

IMViC is an acronym that stands for four different tests; indole test, methyl red test, Voges-Proskauer test, and citrate utilization test.

MUG  (Beta-Glucuronidase) Test for E. coli

MUG (Beta-Glucuronidase) Test for E. coli

Escherichia coli produces the enzyme beta-D-glucuronidase, which hydrolyzes a substrate giving blue fluorescence.

Triple Sugar Iron (TSI) Agar: Principle, Results, and Interpretation

Triple Sugar Iron (TSI) Agar: Principle, Results, and Interpretation

TSI test determines whether a gram-negative rod utilizes glucose, and lactose or sucrose fermentatively and forms hydrogen sulfide.

Shigella: Disease, Properties, Pathogenesis, Lab Diagnosis

Shigella: Disease, Properties, Pathogenesis, Lab Diagnosis

The causative agent of Bacillary dysentery, Shigella is a member of family Enterobacteriaceae. It is non-motile, non-lactose fermenter.

Citrate Utilization Test: Principle, Procedure, Results

Citrate Utilization Test: Principle, Procedure, Results

Citrate test determines the ability of an organism to utilize sodium citrate as source source of carbon.

Biochemical Tests for Bacterial Identification

Biochemical Tests for Bacterial Identification

Catalase test, oxidase test, MUG test, optochin sensitivity test, bacitracin sensitivity test, coagulase test, etc are some of the common biochemical tests.

Salmonella: Properties, Disease, Lab Diagnosis

Salmonella: Properties, Disease, Lab Diagnosis

Escherichia coli (E. coli): Properties and Identification

Escherichia coli (E. coli): Properties and Identification

Normal flora of GI tract, E.coli is a major cause of urinary tract infections. It is lactose fermenting, gram negative bacilli.

Indole Test: Principle, Procedure, Results

Indole Test: Principle, Procedure, Results

Indole test determines the ability of an organism to split tryptophan to form indole.