Methyl Red (MR) test determines whether the microbe performs mixed acids fermentation when supplied glucose. Types and proportion of fermentation products produced by anaerobic fermentation of glucose is one of the key taxonomic characteristics which […]
Catalase is an enzyme, which is produced by microorganisms that live in oxygenated environments to neutralize toxic forms of oxygen metabolites; H2O2. The catalase enzyme neutralizes the bactericidal effects of hydrogen peroxide and protects them. […]
Enterobacteriaceae family contains a large number of genera that are biochemically and genetically related to one another. Many of the traditional or familiar bacteria are found in this family e.g. Escherichia, Shigella, Salmonella, Enterobacter, Proteus, […]
Each of the letters in “IMViC” stands for one of these tests. “I” is for indole; “M” is for methyl red; “V” is for Voges-Proskauer, and “C” is for citrate, lowercase “i” is added for […]
MUG is an acronym for 4-methylumbelliferyl-beta-D-glucuronide. Escherichia coli produces the enzyme beta-D-glucuronidase. Beta-D-glucuronidase is a enzyme which hydrolyzes the beta-D-glucopyranosid-uronic derivatives to aglycons and D-glucuronic acid. The substrate 4-methylumbelliferyl-beta-D-glucuronide is impregnated in the disc and is hydrolyzed […]
Whenever you see the name of this test i.e. Triple Sugar Iron Agar, you have to remember that it’s a test which has three sugar (Lactose, Sucrose, and Glucose) and also iron; and it contains Agar […]
Shigellae are Gram-negative, non-motile, non-spore forming, rod-shaped highly infectious bacteria of the Enterobacteriaceae family. Shigella species include; Shigella sonnei, S. boydii, S. flexneri, and S. dysenteriae. Classification Shigella species are classified by four species (serogroups): Serogroup A: Shigella dysenteriae (12 serotypes) […]
Citrate utilization test is commonly employed as part of a group of tests, the IMViC (Indole, Methyl Red, VP and Citrate) tests, that distinguish between members of the Enterobacteriaceae family based on their metabolic by-products. Citrate […]
In this blog post, i am sharing information about common, conventional biochemical tests and their primary uses in Microbiology laboratory for the identification of particular pathogen of interest. Some of the biochemical tests mentioned here […]
Salmonella is a Gram-negative, rod-shaped, motile bacilli which moves with the use of its peritrichous flagella. The genus Salmonella can be divided into two species (S. enterica and S. bongori), based on their phenotypic profile.The […]
Escherichia coli (abbreviated as E. coli) is a commensal (normal flora) of gut of humans and warm-blooded animals. Most strains of E.coli are harmless, some even benefit the hosts by producing vitamin K in the […]
Indole test is used to determine the ability of an organism to split amino acid tryptophan to form the compound indole. Tryptophan is hydrolysed by tryptophanase to produce three possible end products – one of which […]
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