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Enterobacteriaceae

Yersinia pestis, major features
Bacteriology

Yersinia pestis: Properties, Disease and Laboratory Diagnosis

April 24, 2020 Acharya Tankeshwar 0

Yersinia pestis is gram-negative rods responsible for highly fatal zoonotic disease, plague. Plague is one of the greatest killers known to mankind with at least three pandemics reported in history. It is transmitted to humans […]

Biochemical tests in Microbiology

Methyl Red (MR) test: Principle, procedure and results

January 24, 2014 Acharya Tankeshwar 10

Methyl Red (MR)  test determines whether the microbe performs mixed acids fermentation when supplied glucose. Types and proportion of fermentation products produced by anaerobic fermentation of glucose are one of the key taxonomic characteristics which […]

Biochemical tests in Microbiology

Catalase test: Principle, Procedure, Results and Applications

October 7, 2013 Acharya Tankeshwar 33

Catalase is an enzyme, which is produced by microorganisms that live in oxygenated environments to neutralize toxic forms of oxygen metabolites; H2O2.  The catalase enzyme neutralizes the bactericidal effects of hydrogen peroxide and protects them. […]

Bacteriology

Enterobacteriaceae Family: Common Characteristics

October 1, 2013 Acharya Tankeshwar 10

Enterobacteriaceae family contains a large number of genera that are biochemically and genetically related to one another. Many of the traditional or familiar bacteria are found in this family e.g. Escherichia, Shigella, Salmonella, Enterobacter, Proteus, […]

IMViC Test results of Escherichia coli
Bacteriology

IMViC Tests: Principle, Procedure and results

July 21, 2013 Acharya Tankeshwar 23

Each of the letters in “IMViC” stands for one of these tests. “I” is for indole; “M” is for methyl red; “V” is for Voges-Proskauer, and “C” is for citrate,  lowercase “i” is added for […]

Bacteriology

MUG (beta-Glucuronidase) test for rapid identification of E. coli

July 20, 2013 Acharya Tankeshwar 3

MUG is an acronym for 4-methylumbelliferyl-beta-D-glucuronide. Escherichia coli produces the enzyme beta-D-glucuronidase. Beta-D-glucuronidase is a enzyme which hydrolyzes the beta-D-glucopyranosid-uronic derivatives to aglycons and D-glucuronic acid. The substrate 4-methylumbelliferyl-beta-D-glucuronide is impregnated in the disc and is hydrolyzed […]

TSI Agar Test results
Biochemical tests in Microbiology

Triple Sugar Iron Agar (TSI): Principle, Procedure and Interpretation

July 16, 2013 Acharya Tankeshwar 82

Whenever you see the name of this test i.e. ‘Triple Sugar Iron Agar‘, you have to remember that it’s a test which has three sugar (lactose, sucrose, and glucose) and also iron; and it contains agar […]

Shigella
Bacteriology

Shigella: Disease, properties, pathogenesis and laboratory diagnosis

May 18, 2013 Acharya Tankeshwar 2

Shigellae are Gram-negative, non-motile, non-spore forming, rod-shaped highly infectious bacteria of the Enterobacteriaceae family. Shigella species include; Shigella sonnei, S. boydii, S. flexneri, and S. dysenteriae. Classification There are 4 major subgroups and 43 recognized serotypes of […]

Bacteriology

Citrate Utilization Test: Principle, Procedure and Results

May 17, 2013 Acharya Tankeshwar 9

Citrate utilization test is commonly employed as part of a group of tests, the IMViC (Indole, Methyl Red, VP and Citrate)  tests, that distinguish between members of the Enterobacteriaceae family based on their metabolic by-products. […]

Overview of Biochemical tests for differentiating Gram positive cocci
Biochemical tests in Microbiology

Overview of Biochemical tests used to identify bacteria in Microbiology laboratory

May 7, 2013 Acharya Tankeshwar 36

In this blog post, i am sharing information about common, conventional biochemical tests and their primary uses in Microbiology laboratory for the identification of particular pathogen of interest. Some of the biochemical tests mentioned here […]

Bacteriology

Salmonella: Properties, Disease and Laboratory diagnosis

April 27, 2013 Acharya Tankeshwar 1

Salmonella is a Gram-negative, rod-shaped, motile bacilli which move with the use of its peritrichous flagella. The genus Salmonella can be divided into two species (S. enterica and S. bongori), based on their phenotypic profile. […]

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Bacteriology

Escherichia coli:Disease, Properties, Pathogenesis, and Laboratory diagnosis

April 27, 2013 Acharya Tankeshwar 3

Escherichia coli (abbreviated as E. coli) is a commensal (normal flora) of gut of humans and warm-blooded animals. Most strains of E.coli are harmless, some even benefit the hosts by producing vitamin K in the […]

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