Klebsiella pneumoniae is a Gram-negative rod shaped bacteria of genus Klebsiella and family Enterobacteriaceae. They are members of normal intestinal flora of humans and animals and may be isolated from a variety of environmental sources.
K. pneumoniae was first isolated in the late 19th century and was initially known as Friedlander’s bacterium
- Non-spore-forming rods
- Facultative anaerobes
- Catalase Test: Positive
- Oxidase Test: Negative
- Lactose fermenter (forms pink colored colonies in MacConkey Agar).
- Presence of polysaccharide capsule (in the culture plate mucoid colonies are seen).
- Non-motile (Klebsiella species are nonmotile and nonflagellated and thus have no H antigens).
Virulence Factors of K. pneumoniae
- Cell wall receptors
- Lipopolysaccharide (endotoxin)
Virulence factors of Klebsiella pneumoniae will be discussed in-depth in another post.
Laboratory diagnosis of K. pneumoniae infection
Sample: Sputum (Red currant-jelly sputum may be seen in patient infected with K.pneumoniae), mid-stream urine or blood (depending on the suspected illness/clinical presentation).
Colony characteristics of Klebsiella pneumoniae
- Blood Agar: Mucoid, non-hemolytic colonies
- MacConkey Agar: Mucoid, lactose-fermenting (pink colored) colonies
Various biochemical tests using conventional methods or miniature commercial system (API-20E or E
|Indole Production Test||Negative (K.oxytoca is Indole positive)|
|Citrate Utilization Test||Positive|
| Hydrogen Sulfide Production |
|Urea Hydrolysis Test||Positive|
|Lysine Decarboxylase Test||Positive|
|Arginine Dihydrolase Test||Negative|
|Ornithine decarboxylase test||Negative|
|Motility at 36 °C||Non-motile|
|Acetate Utilization Test||Positive|
Note: To minimize the cost and effort, some diagnostic/hospital laboratories perform only certain tests such as Triple Sugar Iron Agar (TSI), Sulphite Indole Motility (SIM) / Urease Indole Motility (UIM) and Citrate utilization test to identify isolates of Enterobacteriaceae family, full panel/commercially available miniature test system (API-20E and Enterotube test) is used only when the tests results are inconclusive.