Gene Transfer Mechanism in Bacteria and It’s types

Gene Transfer Mechanism in Bacteria

In bacterial population DNA can be transferred from one organism to another by the horizontal transfer mechanism (apart from vertical inheritance). The DNA thus transferred by lateral/horizontal method can be stably incorporated in the recipient , and changes the genetic composition of recipient permanently.

Gene Transfer Mechanism in Bacteria
Gene Transfer Mechanism in Bacteria

Three broad mechanisms mediate efficient movement of DNA between cells- conjugation, transduction and transformation.

  1. Conjugation: Transfer of genes between cells that are in physical contact with one another
  2. Transduction: Transfer of genes from one cell to another by a bacteriophage
  3. Transformation: Transfer of cell-free or “naked” DNA from one cell to another

Conjugation:

  • Conjugation was the first extensively studied method of gene transfer
  • Conjugation requires donor cell-to-recipient cell contact and is mediated by sex pilus
  • Process occurs between two living cells
  • Requires mobilization of donor bacterium’s chromosome/ plasmid
  • Plasmid are genetic elements most frequently transferred by conjugation

Transduction

  • Phage mediated genetic recombination in bacteria i.e. phage is used to transfer DNA from one bacterium to another
  • Transducing particle: Bacterial nucleic acid in phage coat
  • There are two broad categories of Transduction
    • Generalized transduction: Where virtually any genetic marker can be transferred
    • Specialized transduction: Bacterial DNA who are adjacent to viral DNA  in the prophage get transferred
  • For artificial Genetic recombination purpose Temperate phage are preferred vehicle for gene transfer
  • Transduction has been found to occur in a variety of bacterial populations including:
    • Escherichia coli
    • Pseudomonas spp
    • Salmonella spp
    • Staphylococcus spp

Transformation

  • Recipient cell uptake free DNA released into the environment.
  • DNA is released it to the environment when another bacterial cell (i.e. donor) dies and undergoes lysis
  • Not all bacteria are able to go for transformation, only some bacteria are able to take free DNA and are able to go transformation. These type of bacterial are called competent bacteria.
  • Pathogenic bacteria showing competence
    • Haemophilus spp
    • Streptococcus spp
    • Neisseria spp

About tankeshwar 365 Articles
Hello, thank you for visiting my blog. I am Tankeshwar Acharya. Blogging is my passion, I am working as a Asst. Professor and Microbiologist at Department of Microbiology and Immunology, Patan Academy of Health Sciences, Nepal. If you want me to write about any posts that you found confusing/difficult, please mention in the comments below.

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