Dermatophytes: Tinea and Lab Diagnosis

Dermatophytes are keratinophilic and keratinolytic fungi. They can affect all keratinized body areas (hair, skin, and nails) and cause cutaneous mycosis (dermatophyte infection or dermatophytosis).

Dermatophytosis spreads by direct or indirect contact with the infected animal or humans. Indirect transfer can occur from the swimming pool floor, showers, brushes, etc. Direct transfer can occur by the viable fungus in the keratin fragments of skin, hair, and nails. The three clinically significant dermatophytes are Trichophyton, Microsporum, and Epidermatophyton. 

  1. The genus Trichophyton is capable of invading the hair, skin, and nails.
  2. The genus Epidermophyton involves the skin and nails only (not hairs).
  3. The genus Microsporum involves only the hair and the skin (not nails).

Mnemonics to remember which dermatophyte does not affect what:

Trichophyton: Infects all three (so Tri)– i.e. Skin, Nail and Hair

Epidermophyton does not affect Hair (REMEMBER “E” is close to ‘H’ alphabetically and it does not infect near ones)

Microsporum does not affect Nails as ‘M’ is close to ‘N’ (similar scenario of not infecting neighbors)

Dermatophytes cause Tinea or ringworm infections. The common symptoms of dermatophytes are irritation, erythema (redness of the skin), edema (swelling), and vesiculation (formation of the vesicle or cyst). In the Tinea pedis, infection is mainly confined to the toe’s clefts. An infected nail gets discolored, thickened, raised, and friable (easily reduced to powder). Most nail infections are due to the T. rubrum.

Classification of Dermatophytes

Dermatophytes are classified into three genera based on the conidia’s morphology and formation: Trichophyton (T), Microsporum (M), and Epidermatophyton (E). Conidia is the structure of asexual reproduction. Many dermatophyte species produce two types of asexual spores: multi-celled macroconidia and single-celled microconidia (arthroconidia).

Conidia of dermatophytes
Conidia of dermatophytes

Dermatophytes are classified into three groups based on their habitat: soil (geophilic species), animals (zoophilic species), and man ( anthropophilic species).

  • Anthropophilic organisms: T. rubrum, T.interdigitale, T. tonsurans, T. violaceum, M.audouinii, M. ferrugineum.
  • Zoophilic organisms: M. canis ( originating from cats and dogs), M. nanum (originating from pigs), T.equinum (originating from horses), T. verrucosum ( originating from cattle)
  • Geophilic organisms: M. gypseum, M. fulvum

Pathogenesis of Dermatophytes

Virulence factors of dermatophytes are arthroconidium (asexual spores), enzymes (keratinase, protease, lipase), and mannan of the fungal cell wall.


Arthroconidium (asexual spores produced by dermatophytes) adheres to the surface of the tissue. Trichophyton rubrum adheres to the epithelial cells by the carbohydrate-specific adhesions, which are expressed on the surface of the spores. Fibrillar projections have been observed in the T. mentagrophytes during the adherence phase. At the skin surface, long and sparse (thin) fibrils connect fungal arthroconidia to keratinocytes.

The arthroconidium then germinates into hypha, and hypha enters into the stratum corneum (the outermost layer of the epidermis). In the pathogenesis of dermatophytoses, the initial interaction between the arthroconidia and the stratum corneum occurs 3-4 hours after contact.


After the adherence, dermatophytes need to obtain the nutrients for their survival. To use the nutrients, the proteins, starch, cellulose, and lipids are degraded into smaller compounds. Dermatophytes then secrete various enzymes like proteases, lipases, elastases, collagenases, phosphatases, and esterases to degrade the complex compounds into simple ones. Keratinases degrade the keratin present in the host tissues into oligopeptides or amino acids. Proteolytic enzymes degrade the proteins of the skin. It helps in the penetration of the stratum corneum.

Development of host response

Dermatophytes have mannan, which suppresses the inflammatory response. It also inhibits the proliferation of the keratinocytes, which allows for establishing the persistent infection.

Clinical features 

Dermatophytes cause dermatophytosis, which is also known as the tinea or ringworm. The clinical presentation of the infection depends on the species, strain of fungus, size of the inoculum, anatomical site, and immune status of the host.

fungal infection
Tinea (ringworm)

1. Tinea capitis

Tinea capitis is the infection of the shaft of scalp hairs caused by the fungus Trichophyton spp. and Microsporum canis. The infection starts on the scalp, where the dermatophytes grow down the keratinized wall of the hair follicles. The infected hair appears dull and grey. T. capitis presents two clinical forms: inflammatory and non-inflammatory.

  • Inflammatory: Kerion, favus, and agminate folliculitis
  • Non-inflammatory: Black dot, seborrheic dermatitis-like, and grey patch


It is the severe form of dermatophytosis which causes deep, suppurative lesions on the scalp. Follicles may be seen with the discharging pus. It is usually caused by the T. verrucosum and T. mentagrophytes, zoophilic dermatophytes. In children, one of the common clinical forms of tinea infection is kerion celsi.


The word favus is derived from the Latin word favus which means the honeycomb-like appearance. Favus is caused by T. schoenleinii, and it forms the cup-like crusts around the infected follicles. The fungal growth occurs within and around the hair follicle, which produces waxy, honeycomb-like crusts on the scalp. It may lead to alopecia (loss of hair) and scarring.

Black dot
Black dot is usually caused by the T. tonsurans and T. violaceum. It attacks the hair shaft by endothrix type invasion with the abundant sporulation inside the hair and breakage of the hair near the surface of the scalp. It results in a black-dot appearance within an area of smooth scalp surface.

2. Tinea corporis

Tinea corporis is caused by Trichophyton rubrum. It is the infection of the body’s glabrous (non-hairy) skin and is caused by tinea glabrosa or tinea glabrata circinata. It is characterized by erythematous scaly lesions and annular, sharply marginated plaques with a raised border that may be single, multiple, or confluent.

3. Tinea pedis (Athlete’s foot)

Tinea pedis is also known as the athlete’s foot because it is seen in individuals who wear shoes for a long period. The warmth and the moisture facilitate the establishment of the fungus.

It causes the infection of the foot, toes, and interdigital web spaces. In the toe webs, scaling, fissuring, maceration, and erythema are associated with the itching and burning sensation. The small vesicles rupture and the thin fluid is discharged. Then there are the chances of secondary infection as the skin will become macerated and peel. Lymphangitis and lymphadenitis will occur in the case of secondary infection.

Further, it will cause the Moccasin or Sandal ringworm. In this condition, the infection extends from the sole to the side of the foot. It is also known as the one-hand-two feet syndrome because it causes infection in the one-hand and two feet of the patient. In the chronic infection, the sole becomes hyperkeratotic, often covered by fine scales. The causative agent of the athlete’s foot is Trichophyton rubrum, Trichophyton mentagrophytes, and Epidermatophyton floccosum. Tinea pedis is divided into four types: interdigital, hyperkeratotic, ulcerative, and vesicular. 

4. Tinea barbae

Tinea barbae is the ringworm infection of the beard and mustache area of the face. It is also called barber’s itch or tinea sycosis. In this condition, erythematous patches can be seen on the face, which shows scaling, fragile, lusterless hairs. It has got a tendency to develop folliculitis. The main causative agent of the Tinea barbae are Trichophyton verruosum,Trichophyton mentagrophytesMicrosporum canis and T. rubrum are occasionally found in it. 

5. Tinea manuum

Tinea manum is the palm’s ringworm infection caused by T. rubrum, T. mentagrophytes, and Epidermatophyton floccosum.

6. Tinea unguium

Tinea unguium is the dermatophyte infection of the nail, caused by T. rubrum, T. mentagrophytes, and Epidermatophyton floccosum

Causative agents

Tinea is caused by the dermatophytes, which include the fungus of the genus Trichophyton, Microsporum, and Epidermatophyton.

Trichophyton spp.

Trichophyton grows only within dead keratinized tissue. It infects skin, hair, and nail. It produces the keratinolytic protease enzyme, which facilitates the entry of the fungus into the living cells. ( The important trichophyton species are Trichophyton rubrum, T. mentagrophytes, T. tonsurans, T. schoneleinii, T. violaceum, T. verrucosum. The Trichophyton rubrum does not perforate the hair in vitro and does not produce urease enzyme, whereas T. mentagrophytes perforates the hair in vitro and produce urease enzyme. 

Epidermatophyton spp.

Epidermatophyton floccosum is the causative agent of Tinea cruris and Tinea pedis. It doesn’t invade the hair and grows in the epidermis, i.e., skin and nails. It doesn’t produce the microconidia, whereas another dermatophyte, Trichophyton, and Microsporum produce both the microconidia and macroconidia. Epidermatophyton produces only macroconidia. 

Epidermatophyton floccosum
Epidermatophyton floccosum

Microsporum spp.  

Microsporum infects the skin and hair but not the nail. The three species of Microsporum that is mostly pathogenic are Microsporum audouinii, M canis, and M gypseum. Rice grain test is the specific test for Microsporum spp. Microsporum spp. primarily grow on the surface of hair shafts (ectothrix), whereas Trichophyton spp. may grow inside the hair shafts (endothrix) and makes the hair fragile and breaking it or at the surface of the hair follicles.

Laboratory diagnosis of Dermatophytes 

Clinical observation and laboratory investigation play a crucial role in diagnosing fungal infection. The sample of choice are the skin, nail, and plucked hairs of short length from the involved sites

1. Collection of samples

Hair, nail, and skin samples are taken in a folded square of black paper or the card. When paper is used, it helps to dry out the specimens preventing them from bacterial contamination. It helps to store the fungus for 12 months without losing its viability.


For the hair sample collection, instead of cutting, hair is plucked from the edge of the lesion


For the skin sample collection, it needs to be washed well and then scraped from the margin of the lesion onto the folded black paper.


Nails scrapping is taken from the nail bed or infected areas after discarding the outer layers.


It includes the hair stubs, the contents of plugged follicles, and skin scales.

Hairbrush sampling

Use the sterile plastic brush and brush it on the scalp. Then inoculate the plastic brush by pressing it in the agar suitable culture media.

2. Wood’s Lamp Examination

It is a device used to diagnose and manage superficial cutaneous fungal infections. It is used for the examination of the scalp and ringworm infection. Most of the infected hair fluorescence when exposed to ultraviolet light. Microsporum audouinii and Microsporum canis fluorescens bright yellow-green, whereas Microsporum gypseum does not fluorescens. Non-fluorescent fungi like T. tonsurans and T. verrucosum are difficult to detect. In Wood’s lamp, positive fluorescence occurs in infected hairs due to using a chemical substance called pteridine. Sometimes false positives may occur if other substances also consist of pteridine. An error may occur when the ointments containing petroleum, jelly, scales, serum, exudates, lint, and dried soaps fluorescens bluish or purple. 

Microorganisms Fluorescence Color
Microsporum audouinii Bright-green
Microsporum canis Bright-green
Microsporum ferrugineum Blue-green
Microsporum distortum Blue-green
Microsporum gypseum Dull-yellow
Trichophyton schoenleinii Dull-green
Malassezia furfur Golden-yellow
Corynebacterium minutissimum Coral-red
Wood’s Lamp examination

3. Direct microscopy

Place the specimen on a clean, grease-free slide and add 10-20% KOH. Cover it with the cover slip and examine it after 20 minutes. In the skin or nails, branching arthrospores are seen. In the hair, Trichophyton spp. forms parallel rows of spores outside (ectothrix) or inside (endothrix) of the hair shaft. T. tonsurans and T. violaceum show the endothrix type of hair invasion. T. mentagrophytes show ectothrix type of hair invasion. Hairs infected with the T. schoneleinii show hyphae and air spaces within the shaft.

Read more from this link: KOH wet mount

4. Fungal Culture

The suitable media for the fungal culture is Potato Dextrose Agar (PDA) or Sabouraud’s dextrose agar (SDA) used for culture. Incorporating antibiotics like gentamicin and chloramphenicol in Sabouraud’s media will inhibit the overgrowth of bacteria. Incorporating the cycloheximide will inhibit the overgrowth of the rapidly growing saprophytic fungi. Sabouraud peptone-glucose agar (Emmons’ modification) mixed with cycloheximide and chloramphenicol is commonly used. Its commercial names are Mycobiotic and Mycosel agars. Inoculation is done at 25-30℃ for 1-3 weeks. The fungi are identified based on colonial appearance, color, pigment production, and microscopic observation. For the microscopic observation, lactophenol cotton blue staining is performed.

Morphological Characteristics of Dermatophytes

Colony morphology of Trichophyton and Microsporum
Colony of Trichophyton and Microsporum
Dermatophytes Colony morphology Microscopic observation
Trichophyton rubrum Velvety, red pigment on reverse Tear-drop, microconidia, a few long, pencil-shaped macroconidia
Trichophyton mentagrophytes White to tan, cottony or powdery, pigment variable Clusters of microconidia cigar-shaped macroconidia with terminal rat-tail filaments
Trichophyton tonsurans Powdery to cream or yellow with central furrows Abundant microconidia, thick-walled, irregular macroconidia
Trichophyton schoenleinii Smooth, waxy, brownish Hyphal swellings, chlamydopsores, favic chandelier
Trichophyton violaceum Very slow growing, waxy, violet to purple pigment Distorted hyphae, conidia rare
Microsporum audouinii Velvety, brownish, slow growing Thick-walled chlamydospores, conidia rare and irregular
Microsporum canis Cottony, orange pigment on reverse Abundant, thick-walled spindle-shaped macroconidia with up to 15 septa
Microsporum gypseum Powdery, buff-colored Yellowish-green Abundant, thin-walled macroconidia with 4-6 septa
Epidermatphyto floccosum Slow growing, powdery, greenish-brown Club-shaped macroconidia in clusters
Microscopic observation of Trichophyton and Microsporum
Microscopic observation of Trichophyton and Microsporum

5. Rice Grain Test

rice grain test
Rice grain test showing growth of Microsporum canis, Image source: The University of Adelaide

Rice grain test differentiates the M. audouinni from the other Microsporum species. M. audouinii doesn’t grow in the presence of rice grain. Sterile non-fortified rice is inoculated lightly with the hyphae of the test isolate. Then after 10 days of incubation at room temperature, the medium is observed for growth. M. canis and other dermatophytes grow well and usually form many conidia, but M. audouinii does not grow. 

6. Dermatophyte Test Medium (DTM)

Dermatophyte Test Medium is used for the presumptive identification of dermatophytes from the other fungal or bacterial contaminants. It helps to distinguish whether the cutaneous lesion is caused by dermatophytes, other fungi, or bacteria. When incubated at 25℃, the dermatophytes turn the medium red, whereas bacteria and other fungi cannot change it. To avoid the false positive result caused by DTM, dermatophyte identification medium (DIM) is also used. 

7. Hair Perforation Test

hair perforation test
Positive hair perforation test, Image source: The University of Adelaide

Hair Perforation is the differential test to differentiate the morphologically similar fungal species. It is used to distinguish the Trichophyton rubrum and Trichophyton mentagrophytes. Trichophyton rubrum causes the surface erosion of the hair shaft, whereas Trichophyton mentagrophytes cause wedge-shaped perforation. The hair perforation test also differentiates the Microsporum canis and Microsporum equinum. Microsporum canis perforates the hair, but the Microsporum equinum does not perforate.

8. Urea hydrolysis test

Incubation is done for 2-3 days on Christensen’s Urea Agar. It is used to differentiate between Trichophyton rubrum and Trichophyton mentagrophytes. When the urea is hydrolyzed, the media turns deep red. T. mentagrophytes shows positive results as it produces urease enzyme within 2-4 days, whereas T. rubrum shows negative results.


For most skin infections, topical therapy is satisfactory. Oral antifungals are required to treat nail and scalp infections and severe or extensive skin infections. Topical agents include azole compounds, terbinafine, amorolfine, and ciclopirox olamine. Oral griseofulvin is beneficial for scalp, skin, and fingernail infections. Terbinafine and itraconazole have largely replaced griseofulvin for treating nail infections because of their much better cure rates and shorter treatment periods.

Prevention and Control 

  • Routine inspection of the scalps should be done, and avoid sharing of combs and brushes.
  • Barbershop instruments ( combs, brushes, and scissors) must be disinfected.
  • Infected clothing, towels, and bedding, these items need to be disinfected after use because there is a chance of transmission of the Tinea corporis and Tinea cruris.
  • Prevention of the tinea pedis can be enhanced by using good foot hygiene.


  1. Brasch, J., Müller, S., & Gräser, Y. (2015). Unusual strains of Microsporum audouinii causing tinea in Europe. Mycoses, 58(10), 573–577.
  2. GREGORY., P. H. (1935). the Dermatophytes. Biological Reviews, 10(2), 208–233.
  3. Su, H., Packeu, A., Ahmed, S. A., Al-Hatmi, A. M. S., Blechert, O., Ilkit, M., Hagen, F., Gräser, Y., Liu, W., Deng, S., Hendrickx, M., Xu, J., Zhu, M., & De Hoog, S. (2019). Species distinction in the Trichophyton rubrum complex. In Journal of Clinical Microbiology (Vol. 57, Issue 9).
  4. Chander, J. (2018). Textbook of Medical Mycology (Fourth edition). Jaypee Brothers Medical Publishers Ltd.
  5. profile, V. (2022). Trichophyton rubrum. Retrieved 21 August 2022, from

Sushmita Baniya

Hello, I am Sushmita Baniya from Nepal. I have completed M.Sc Medical Microbiology. I am interested in Genetics and Molecular Biology.

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