Culture Media

Culture media are a combination of pre-defined ingredients that support the growth and proliferation of microorganisms in an artificial/in vitro environment. They are used for the isolation of organisms from various samples such as clinical specimens, food, or the environment.

Based on the consistency, culture media are further categorized as solid, semisolid, and liquid (broth). Functionally, culture media can be classified as differential, selective, enriched, enrichment, assay, transport, etc.

Anaerobic Bacteriology

Thioglycollate broth

Nisha Rijal 8

Last updated on June 21st, 2021Thioglycollate broth is the enrichment broth most frequently used in diagnostic bacteriology. This broth supports the growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms.  It contains many nutritive factors, including […]

Culture Media

Alkaline Peptone Water (APW)

Acharya Tankeshwar 3

Last updated on July 1st, 2021Alkaline peptone water (APW) is recommended as an enrichment broth for isolating Vibrio cholerae from clinical samples and non-clinical samples (suspected food & water samples). A number of other broth media have been […]

Culture Media

Salmonella-Shigella (SS) Agar

Acharya Tankeshwar 7

Last updated on June 21st, 2021Salmonella-Shigella (SS) agar is used for selective isolation and differentiation of Salmonella and Shigella. It is used for the isolation, cultivation and differentiation of gram-negative enteric microorganisms from both clinical […]

Culture Media

Baird Parker Agar: Preparation, Uses

Nisha Rijal 0

Last updated on June 17th, 2021Baird Parker Agar was developed by Baird Parker in 1962 and is a modification of the Tellurite-glycine formulation of Zebovitz et al. It is a moderately selective medium for the […]

Culture Media

Xylose Lysine Deoxycholate (XLD) Agar

Nisha Rijal 3

Last updated on June 24th, 2021Xylose lysine deoxycholate (XLD) agar is both a selective and differential medium for the isolation, cultivation and differentiation of gram-negative enteric microorganisms. This media is primarily used for isolation and […]