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HomeCulture Media used in Microbiology

Culture Media used in Microbiology

Bacteriology

Tryptic Soy Agar (TSA): Composition, Preparation and Uses

February 9, 2018 Acharya Tankeshwar 9

Tryptic Soy Agar is a universal medium which supports the growth of wide variety of microorganisms (both gram positive as well as gram negative bacteria grow in this medium).  It is used for the isolation […]

Bacteriology

Cary-Blair transport medium: Composition, Preparation and Uses

September 10, 2016 Acharya Tankeshwar 3

Cary-Blair transport medium can be used to transport of clinical specimens suspected to contain enteric pathogens, including  Shigella, Salmonella, Vibrio cholerae, and Escherichia coli O157:H7. Since this transport medium has a high pH (8.4), viability […]

Bacteriology

Alkaline peptone water (APW): Principle, Preparation and Uses

September 10, 2016 Acharya Tankeshwar 3

Alkaline peptone water (APW) is recommended as an enrichment broth for isolating Vibrio cholerae from clinical samples and non clinical samples (suspected food & water samples). A number of other broth media have been described for enrichment of […]

Bacteriology

Modified Thayer-Martin Agar: Composition, preparation, uses and colony characteristics

June 13, 2016 Acharya Tankeshwar 3

Thayer-Martin agar medium is a selective medium used for the isolation of gonococci (Neisseria gonorrheae)  from specimen containing a mixed flora of bacteria and/or fungi e.g. urogenital specimen. Modified Thayer-Martin (MTM) agar is a GC agar […]

Nutrient Agar
Bacteriology

Nutrient Agar: composition, preparation and uses

March 12, 2016 Acharya Tankeshwar 15

Nutrient Agar is used as a general-purpose medium for the growth of a wide variety of non-fastidious microorganisms. It consists of peptone, beef extract, and agar. This relatively simple formulation provides the nutrients necessary for the replication of a […]

Culture Media used in Microbiology

Potato Dextrose Agar (PDA): principle, composition and colony characteristics

June 1, 2015 Nisha Rijal 6

Potato dextrose agar (PDA) is a general purpose basal medium for the identification, cultivation and enumeration of yeast and molds in foods and dairy products. It may also be used for the cultivation of yeasts […]

Culture Media used in Microbiology

Baird Parker Agar- principle, preparation and uses

May 26, 2015 Nisha Rijal 0

Baird Parker Agar was developed by Baird Parker in 1962 and is a modification of Tellurite-glycine formulation of Zebovitz et al. It is a moderately selective medium for the isolation and differentiation of coagulase positive […]

Culture Media used in Microbiology

Xylose lysine deoxycholate (XLD) agar: Composition Preparation, Results and Uses

April 22, 2015 Nisha Rijal 3

Xylose lysine deoxycholate (XLD)  Agar was formulated by Taylor for the isolation and differentiation of enteric pathogens including Salmonella Typhi from other Salmonella species. XLD Agar is both selective and differential medium for the isolation, […]

Culture Media used in Microbiology

Common Fungal Culture Media and their uses

January 26, 2014 Acharya Tankeshwar 10

Two general types of culture media are essential to ensure the primary recovery of all clinically significant fungi from clinical specimens. One medium should be non-selective (such as Brain Heart Infusion Agar; i.e., one that will permit […]

No Picture
Bacteriology

New York City Medium Agar: Introduction, principle, composition and uses

January 26, 2014 Acharya Tankeshwar 0

New York City (NYC) Medium is primarily designed for isolation of pathogenic Neisseria.  It also supports the growth of genital mycoplasmas (Mycoplasma hominis and Ureaplasma urealyticum). New York City (NYC) medium is useful in the diagnosis […]

Culture Media used in Microbiology

Thiosulfate citrate bile salts sucrose (TCBS) agar: Composition, uses and colony characteristics

September 10, 2013 Acharya Tankeshwar 3

Thiosulfate citrate bile salts sucrose (TCBS) agar is a selective as well as differential culture medium used for selective isolation of  Vibrio spp from a variety of clinical and nonclinical specimens.  Vibrio cholerae is the […]

Bacteriology

Chocolate Agar: Composition, uses and colony characteristics

September 8, 2013 Acharya Tankeshwar 12

Chocolate Agar (CAP) is the lysed blood agar. The name is itself derived from the fact that red blood cell (RBC) lysis gives the medium a chocolate-brown color. Chocolate agar is used for the isolation […]

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