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Category: Bacteriology

Preparation of Antibiotic Stock Solutions and Discs

Preparation of antibiotic stock solutions Antibiotics may be received as powders or tablets. It is recommended to obtain pure antibiotics from commercial sources, and not use injectable...

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link to Transport and Storage of Antimicrobial Discs

Transport and Storage of Antimicrobial Discs

Cartridges containing commercially prepared antimicrobial discs for susceptibility testing are generally packaged to ensure appropriate anhydrous conditions. Temperature and moisture are the...

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link to Satellitism Test for Haemophilus influenzae

Satellitism Test for Haemophilus influenzae

Most species of Haemophilus require both X and V factors for growth. As V factors are released erythrocytes into the medium, it is available for bacteria to utilize. In contrast, hemin is found...

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link to RPR Test: Principle, Procedure, Interpretations

RPR Test: Principle, Procedure, Interpretations

The rapid plasma reagin (RPR) test is a macroscopic, nontreponemal flocculation card test used to screen for syphilis.   Let’s first analyze the test name: Rapid: It is a rapid...

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link to Anti-streptolysin O Titer (ASOT): Principle, Procedure, and Results

Anti-streptolysin O Titer (ASOT): Principle, Procedure, and Results

Most people infected with group A streptococci produce anti-streptolysin O (ASO) antibodies. In infections caused by β-hemolytic streptococci,  streptolysin-O is one of the two hemolytic...

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link to CSF Sample: Collection, Processing, Staining, and Culture

CSF Sample: Collection, Processing, Staining, and Culture

Meningitis is an inflammation of the membranes covering the brain and spinal cord known as the meninges. The most common etiologic agents of acute meningitis are enteroviruses (primarily echoviruses...

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