Preparation of antibiotic stock solutions and dried paper discs

Preparation of antibiotic stock solutions
Antibitiotics may be received as powders or tablets. It is recommended to obtain pure antibiotics from commercial sources, and not use injectable solutions.

Powders must be accurately weighed and dissolved in the appropriate diluents  to yield the required concentration, using sterile glassware.

Standard strains of stock cultures should be used to evaluate the antibiotic stock solution. If satisfactory, the stock can be aliquoted in 5 ml volumes and frozen at -20ºC or -60ºC.

Stock solutions are prepared using the formula (1000/P) X V X C=W, where

  1. P= potency of the antibiotic base,
  2. V=volume in ml required,
  3. C=final concentration of solution and
  4. W=weight of the antimicrobial to be dissolved in V.

Preparation of dried filter paper discs
Whatman filter paper no. 1 is used to prepare discs approximately 6 mm in diameter, which are placed in a Petri dish and sterilized in a hot air oven.
Dispense 0.005 ml (5 microliter) of antibiotic solultion using sterile micropipette tips.

 
About tankeshwar 363 Articles
Hello, thank you for visiting my blog. I am Tankeshwar Acharya. Blogging is my passion, I am working as a Asst. Professor and Microbiologist at Department of Microbiology and Immunology, Patan Academy of Health Sciences, Nepal. If you want me to write about any posts that you found confusing/difficult, please mention in the comments below.

7 Comments

  1. So except for the hot air oven sterilization, which other effective methods can be used to sterilize the filter paper disc?

     
  2. sir i want to check inhibition of S.aureus by using Alovera plant, but i am confused how to make antibiotic discs from Alovera at home.
    Plz gauid me sir
    waiting for kind reply.

     

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