Phenylalanine deaminase test also known as phenylpyruvic acid (PPA) test is used to test the ability of an organism to produce enzyme deaminase. This enzyme removes the amine group from the amino...
Category: Biochemical Tests
Oxidative fermentative (OF) test: Principle, Procedure, Results
The oxidative-fermentative (OF) test was developed by Hugh and Leifson in 1953. They developed OF media to differentiate between oxidative bacteria (that produces acid from carbohydrates under...
Elek test is an in vitro immunoprecipitation (immunodiffusion) test to determine whether or not a strain of Corynebacterium diphtheriae is toxigenic. A test strip of filter paper containing...
The motility test is used to determine whether an organism is motile or non-motile. Motile organisms contain flagella which helps them to travel beyond the point of inoculation. Motile bacteria are...
Lecithinase test or Nagler's reaction is a biochemical test used to identify organisms that liberate phospholipases (lecithinases) e.g. Clostridium perfringens. The alpha (α) toxin of C. perfringens...
Acetate utilization test is used to determine if an organism can use acetate as the sole source of carbon. Acetate agar containing sodium acetate as the sole carbon source and inorganic ammonium...
