The Voges-Proskauer (VP) test determines if an organism produces acetylmethylcarbinol from glucose fermentation. Voges-Proskauer is a double eponym named after two microbiologists working at the...
Category: Biochemical Tests
Decarboxylases are a group of substrate-specific enzymes that are capable of reacting with the carboxyl (COOH) portion of amino acids, forming alkaline-reacting amines and byproduct carbon...
Hippurate hydrolysis test-Left (positive tube), Right (Negative tube) Hippurate hydrolysis occurs in an organism that can form glycine and benzoic acid. In the classical test (48 hours), the...
Tween 80 is the trade name of the detergent polyethylene derivative of sorbitan mono-oleate. Some Mycobacterium species possess an enzyme-lipase, that splits the compound and releases oleic acid and...
ONPG (β-galactosidase) Test: Principle, Procedure and Results
O-Nitrophenyl-β-D-galactopyranoside (ONPG) is structurally similar to lactose (i.e. O-Nitrophenyl-β-D-galactopyranoside is an analog of lactose), except that orthonitrophenyl has been substituted...
Nitrate reduction test is used for the differentiation of members of Enterobacteriaceae based on their ability to produce nitrate reductase enzyme that hydrolyzes nitrate (NO3-) to nitrite...