Biochemical Tests

Nitrocefin test: Principle, Procedure, Uses

Last updated on June 2nd, 2021The nitrocefin biochemical test is a sensitive technique for detecting beta-lactamase-producing strains of N. gonorrhoeae, H. influenzae, Staphylococcus spp, Enterococcus spp, and Moraxella (Branhamella) catarrhalis. Nitrocefin is the only reliable test […]

Biochemical Tests

Voges Proskauer (VP) Test

Last updated on June 21st, 2021The Voges-Proskauer (VP) test is used to determine if an organism produces acetylmethylcarbinol from glucose fermentation. Voges-Proskauer is a double eponym, named after two microbiologists working at the beginning of […]


ONPG: β-galactosidase Test

Last updated on June 11th, 2021O-Nitrophenyl-β-D-galactopyranoside (ONPG) is structurally similar to lactose (i.e. ONPG is an analog of lactose), except that orthonitrophenyl has been substituted for glucose. On hydrolysis, through the action of the enzyme […]