This post was most recently updated on April 30th, 2019Voges-Proskauer is a double eponym, named after two microbiologists working at the beginning of the 20th century. They first observed the red color reaction produced by […]
This post was most recently updated on May 17th, 2017CLED (cysteine-, lactose-, and electrolyte-deficient) agar is a differential culture medium for use in isolating and enumerating bacteria in urine from the suspected cases of Urinary […]
This post was most recently updated on April 14th, 2018Decarboxylases are a group of substrate specific enzymes that are capable of reacting with the carboxyl (COOH) portion of amino acids, forming alkaline-reacting amines and byproduct […]
This post was most recently updated on May 2nd, 2019Hippurate is the glycine conjugate of benzoic acid. When hippurate is hydrolysed by an organism glycine and benzoic acid are formed. In the classical test (48 […]
This post was most recently updated on December 27th, 2018Streptococci are Gram positive cocci either diplococci (lanceolate shaped; S. pneumoniae) or in chains. They are catalase negative (An important test which differentiate it from another […]
This post was most recently updated on April 29th, 2016Tween 80 is the trade name of the detergent polyethylene derivative of sorbitan mono-oleate. Some Mycobacterium species posses an enzyme-lipase, that splits the compound and releases […]
This post was most recently updated on April 30th, 2019O-Nitrophenyl-β-D-galactopyranoside (ONPG) is structurally similar to lactose (i.e. ONPG is an analog of lactose), except that orthonitrophenyl has been substituted for glucose. On hydrolysis, through the […]
This post was most recently updated on April 11th, 2016Nitrate reduction test is used for the differentiation of members of Enterobacteriaceae on the basis of their ability to produce nitrate reductase enzyme that hydrolyze nitrate (NO3–) […]
This post was most recently updated on January 29th, 2015Hektoen Enteric (HE) Agar was introduced in 1968 by Sylvia King and William I. Metzger. They formulated HE Agar medium while working at the Hektoen Institute […]
This post was most recently updated on April 30th, 2019Salmonella is a member of the family Enterobacteriaceae. It is a Gram-negative bacilli, motile and non-lactose fermenter. The genus can be divided into two species (S. […]
This post was most recently updated on March 12th, 2016Principle String Test: When an isolated colony (18-24 hour growth) of a suspected bacterium is emulsified in Sodium deoxycholate or Sodium taurocholate (commonly known as bile […]
This post was most recently updated on April 20th, 2019DNA hydrolysis test or Deoxyribonuclease (DNase) test is used to determine the ability of an organism to hydrolyze DNA and utilize it as a source of […]
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