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Category: General Microbiology

link to SEM vs. TEM Electron Microscopy

SEM vs. TEM Electron Microscopy

The advent of Electron Microscopy in 1932 opens the door to visualizing small subcellular structure and viruses which were beyond the scope of light microscopy which can’t resolve objects separated...

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link to Spread Plate Technique: Principle, Procedure, Results

Spread Plate Technique: Principle, Procedure, Results

The spread plate technique is a viable counting method employed to plate a liquid sample to isolate or count the bacteria in that sample. A perfect spread plate technique will result in visible and...

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link to Pour Plate Method: Procedure, Uses, (Dis) Advantages

Pour Plate Method: Procedure, Uses, (Dis) Advantages

Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. Because the sample is mixed with the molten agar medium, a larger...

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link to Streak Plate Method: Principle, Procedure, Uses  

Streak Plate Method: Principle, Procedure, Uses  

For organisms that grow well on agar plates, a streak plate is the method of choice for obtaining pure culture. The streak plate technique is used to isolate the organisms (mostly bacteria) from a...

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link to Ethylene Oxide (ETO) Sterilization: Features and Limitations

Ethylene Oxide (ETO) Sterilization: Features and Limitations

Ethylene oxide (ETO) has been widely used as a low-temperature sterilant. ETO is a colorless gas that is flammable and explosive but it is liquid at temperatures below 10.8°C. ETO is an...

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link to Robert Koch and Koch’s postulates

Robert Koch and Koch’s postulates

Robert Koch (1843-1910) was a German physician, scientist, and the first person to prove that bacterium is the cause of animal disease. Robert Koch was born on December 11, 1843, at Clausthal in the...

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