Shell vial cell culture, a centrifuge enhanced tissue culture assay, is a modification of conventional cell culture for the rapid detection of viruses in vitro. Principle The technique involves inoculation of the clinical specimen on […]
Radioimmunoassay (RIA) is very sensitive (can detect at a concentration of <0.01 μg/mL) and a specific technique that is used for the quantitative detection of antigens or haptens. RIA is an extremely important tool in […]
When a person is infected with a pathogen, antibodies are produced against many epitopes of this pathogen. A subset of these antibodies can block infection by a process called neutralization. These antibodies block or distort the […]
The indirect fluorescent antibody test (IFA) is a semi-quantitative, sensitive, and rapid test to detect which can be used to detect specific antibodies or antigens present in the samples. IFA for the detection of Antibodies […]
Electron microscope as the name suggests is a type of microscope that uses electrons instead of visible light to illuminate the object. Electromagnets function as lenses in the electron microscope, and the whole system operates […]
Embroyonated chicken eggs are used for the cultivation of some viruses. The viruses grow in the cells of the embryo and membranes. Specimens are inoculated into pathogen-free fertilized eggs of 10-11 days and are incubated […]
Today, the whole world is suffering and threatened with this newly discovered disease, the COVID-19. People who are infected with the coronavirus are showing symptoms that are almost similar to having colds or flu. Sadly, […]
Legionella is a faintly staining gram-negative pleomorphic rod, responsible for causing Legionnaires‘ disease; a severe form of atypical pneumonia, and Pontiac fever. Legionella was first recognized in 1976 after an outbreak of pneumonia at an American […]
Mycobacterium tuberculosis (MTB) is the causative agent of tuberculosis (TB) in humans. It is a slightly curved rod arranged singly or in groups. Mycobacterium tuberculosis grows very slowly, taking up to 6 weeks for visible […]
Fluorescence microscopy is a type of light microscope that works on the principle of fluorescence. A substance is said to be fluorescent when it absorbs the energy of invisible shorter wavelength radiation (such as UV […]
Corynebacterium diphtheriae also known as Klebs-Löffler bacillus is a Gram-positive bacillus responsible for causing diphtheria. Once a major cause of illness and death among children, diphtheria became a rare disease after the administration of vaccination. The […]
Filtration is the preferred method of sterilizing heat sensitive liquid and gases without exposure to denaturing heat. Rather than destroying contaminating microorganisms, it simply removes them. It is the method of choice for sterilizing antibiotic […]
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