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Home2013September

Month: September 2013

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Bacteriology

Weil Felix Test: Principle, Procedure and limitation

September 28, 2013 Acharya Tankeshwar 5

Weil-Felix is a nonspecific agglutination test that detects anti-rickettsial antibodies in patient’s serum. Weil-Felix test is based on cross-reactions that occur between antibodies produced in acute rickettsial infections with antigens of OX (OX  19, OX […]

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Bacteriology

How to collect Midstream Specimen of Urine (MSU)?

September 27, 2013 Acharya Tankeshwar 0

Instructions for female patients to collect midstream urine for bacterial culture Remove undergarments. Wash hands thoroughly with soap and water, rinse them, and dry them on a disposable paper towel or shake off excess water. […]

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Virology

How can cases of viral hemorrhagic fever be prevented and controlled?

September 21, 2013 Acharya Tankeshwar 0

Prophylaxis: Vaccines are available for yellow fever and Argentine hemorrhagic fever that can protect against these diseases to some extent. Prevention of Viral Hemorrhagic Fever can be achieved by either control of rodents, or protecting from bite […]

Bacteriology

Bacterial Transformation: Mechanism

September 21, 2013 Acharya Tankeshwar 1

Bacterial Transformation is one of the three horizontal gene transfer mechanism found in Bacterial cells.  The other two horizontal gene transfer mechanism are conjugation (Transfer of F Plasmid & Transfer of Chromosomal DNA)  and transduction […]

Microbial Genetics

Conjugation: Transfer of Chromosomal DNA by High Frequency Recombination (HFr) Strain

September 12, 2013 Acharya Tankeshwar 2

The F+ strain which contains plasmid gene as an episome (i.e.  F plasmid becomes integrated into the host cell genome at one of the several possible sites chromosomal gene) induce more than thousand times the number of […]

Microbial Genetics

Mechanism of Conjugation in Bacteria: The transfer of F Plasmid

September 12, 2013 Acharya Tankeshwar 1

Conjugation is one the three mechanism of DNA exchange between bacteria, the other being transformation and transduction. Conjugation occurs between two living cells, involves cell to cell contact, and requires mobilization of either a plasmid […]

Microbial Genetics

Gene Transfer Mechanism in Bacteria and It’s types

September 12, 2013 Acharya Tankeshwar 0

In bacterial population DNA can be transferred from one organism to another by the horizontal transfer mechanism (apart from vertical inheritance). The DNA thus transferred by lateral/horizontal method can be stably incorporated in the recipient […]

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Microbial Genetics

Specialized transduction: Mechanism

September 12, 2013 Acharya Tankeshwar 0

In Transduction, DNA is transferred from one cell to another through the agency of viruses.  Genetic transfer of host gene by bacteriophage occurs in two ways-Generalized transduction and specialized transduction. Specialized transduction occurs only in […]

Microbial Genetics

Generalized transduction: Mechanism

September 12, 2013 Acharya Tankeshwar 1

In Transduction, DNA is transferred from one cell to another through the agency of viruses.  Genetic transfer of host gene by bacteriophage occurs in two ways-generalized transduction and specialized transduction. In generalized transduction, virtually any […]

Culture Media used in Microbiology

Thiosulfate citrate bile salts sucrose (TCBS) agar: Composition, uses and colony characteristics

September 10, 2013 Acharya Tankeshwar 3

Thiosulfate citrate bile salts sucrose (TCBS) agar is a selective as well as differential culture medium used for selective isolation of  Vibrio spp from a variety of clinical and nonclinical specimens.  Vibrio cholerae is the […]

Microbiology for Beginners

What are the roles of Medical Microbiologist?

September 10, 2013 Acharya Tankeshwar 4

Microbiologists work in public health laboratories, hospital laboratories, reference or independent laboratories, and physician office laboratories. Microbiologist must be aware of what the physician needs and as well as what the laboratory needs. At an […]

Bacteriology

Colony Morphology of Bacteria; How to describe Bacterial Colonies?

September 8, 2013 Acharya Tankeshwar 11

Bacteria grow on solid media as colonies. A colony is defined as a visible mass of microorganisms all originating from a single mother cell. Key features of these bacterial colonies serve as important criteria for […]

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