Cytopathic effect (CPE) refers to morphological changes in host cells because of viral infection. It may aid in viral disease diagnosis.
Shell vial culture involves inoculation of the clinical specimen on cell monolayer for rapid detection of viruses in vitro.
Embryonated chicken eggs are used for the cultivation of some viruses. The viruses grow in the cells of the embryo and membranes.
Lysis of the bacteriophage is indicated by the formation of a zone of clearing or plaque within the lawn of bacteria.
Cells used to culture are fibroblast, lymphocytes, cells from cardiac and skeletal tissues, cells from liver, breast, skin, kidney, and tumor cells.