Specimens for anaerobic culture should be properly collected and transported. Indigenous anaerobes are often present in large numbers as normal flora on mucosal surfaces (e.g. mouth). So the sample from sites known to have anaerobes as part of the normal flora is unacceptable for anaerobic culture.
Specimen for anaerobic culture is best obtained by tissue biopsy or by aspiration using a needle and syringe. Generally following clinical specimen are suitable for anaerobic culture
- Biopsy of endometrial tissue obtained with an endometrial suction curette
- Bone marrow
- Branchial washings obtained with double lumen plugged catheter
- Cerebrospinal fluid
- Culdocentesis aspirate
- Decubitus ulcer, if obtained from the base of the lesion after thorough debridement of surface debris
- Fluid from normally sterile sites (e.g. joint)
- Material aspirated from abscesses (the best specimens are from loculated or walled-off lesions)
- Percutaneous (direct) lung aspirate or biopsy
- Peritoneal (ascitic) fluid
- Sulfur granules from draining fistula
- Suprapubic bladder aspirate
- Thoracentesis (pleural) fluid
- Tissue obtained at biopsy or autopsy
- Transtrancheal aspirate
- Uterine contents if collected using a protected swab
Acharya TankeshwarHello, thank you for visiting my blog. I am Tankeshwar Acharya. Blogging is my passion. As an asst. professor, I am teaching microbiology and immunology to medical and nursing students at PAHS, Nepal. I have been working as a microbiologist at Patan hospital for more than 10 years.
Robertson’s Cooked Meat (RCM) Medium
The most widely used anaerobic culture medium containing chopped meat particles and used for maintenance of stock cultures.