Last updated on May 26th, 2021Gram-positive bacteria retain the color of the primary stain (crystal violet) in the Gram staining procedure and appear as purple/violet under a light microscope. These bacteria have a cell wall […]
Last updated on June 21st, 2021Microbes are loosely classified into several groups based on their preferred temperature ranges. Every organism has a range of permissive growth temperatures. Unlike pH or osmolarity, microbes cannot regulate their […]
Last updated on June 1st, 2021Gram staining requires the use of different chemical reagents which can be purchased in ready-to-use forms from commercial suppliers or can be prepared at the laboratory by mixing different chemicals […]
Last updated on May 30th, 2021Laboratory diagnosis of intestinal parasitic infections can be carried out by detection and identification of the parasites or their particular stages (ova/egg, cyst, larva or trophozoite) in the stool specimen. […]
Last updated on June 21st, 2021Chromogenic culture media are used to isolate, identify, and differentiate specific microorganisms from a heterogeneous population. The medium contains chromogenic substrate which is utilized by the microorganisms to give colored colonies […]
Last updated on June 11th, 2021The advent of Electron Microscopy in 1932 opens the door to visualizing small subcellular structure and viruses which were beyond the scope of light microscopy which can’t resolve objects separated […]
Last updated on June 7th, 2021The target readers of this blog post are general people (people who do not have a health science background) who want to get basic information about “blood culture” as they […]
Last updated on June 11th, 2021Spread plate technique is a viable counting method employed to plate a liquid sample for the purpose of isolating or counting the bacteria present in that sample. A perfect spread plate technique will […]
Last updated on May 29th, 2021Most Probable Number (MPN) is a method used to estimate the concentration of viable microorganisms in a sample by means of replicate liquid broth growth in ten-fold dilutions. It is […]
Last updated on June 21st, 2021The carbohydrate fermentation test is used to determine whether or not bacteria can ferment a specific carbohydrate. Carbohydrate fermentation patterns are useful in differentiating among bacterial groups or species. It tests […]
Last updated on June 21st, 2021Microorganisms can produce different types of volatile compounds that may give characteristics smell, pleasant scent, or pungent odor. Production of these volatile chemicals depends on the metabolic characteristics of that […]
Last updated on June 11th, 2021Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. Because the sample is mixed with the molten agar […]
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