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Home2015January

Month: January 2015

Bacteriology

Nitrate Reduction Test: Principle, Procedure and Results

January 30, 2015 Acharya Tankeshwar 17

Nitrate reduction test is used for the differentiation of members of Enterobacteriaceae on the basis of their ability to produce nitrate reductase enzyme that hydrolyze nitrate (NO3–) to nitrite (NO2–) which may then again be degraded […]

Bacteriology

Hektoen Enteric (HE) Agar: composition, principle and uses

January 27, 2015 Acharya Tankeshwar 0

Hektoen Enteric (HE) Agar was introduced in 1968 by Sylvia King and William I. Metzger. They formulated HE Agar medium while working at the Hektoen Institute in Chicago, to increase the recovery Salmonella and Shigella from clinical specimens. […]

Bacteriology

Culture media used for isolation and identification of Salmonella typhi and paratyphi

January 27, 2015 Acharya Tankeshwar 11

Salmonella is a member of the family Enterobacteriaceae. It is a Gram-negative bacilli, motile and non-lactose fermenter. The genus can be divided into two species (S. enterica and S. bongori), based on their phenotypic profile. […]

String test for Vibrio Cholerae
Bacteriology

String test for Laboratory diagnosis of Vibrio Cholerae

January 23, 2015 Acharya Tankeshwar 0

Principle String Test: When an isolated colony (18-24 hour growth) of a suspected bacterium is emulsified in Sodium deoxycholate or Sodium taurocholate (commonly known as bile salt),it lyses the cell wall of the bacterium releasing […]

Normal Red Blood Cell and Sickle Shaped RBC
Parasitology

Relationship between sickle cell anemia and malaria

January 11, 2015 Acharya Tankeshwar 4

In my undergraduate teaching class, one of the most common queries of student is; “People with sickle cell anemia is protected against malaria”? The above query is only partially right because: Sickle cell hemoglobin confers a survival […]

MIC by E-Test
Antibiotic Resistance

E-TEST ( Epsilometer test): Principle, purpose, procedure, results and interpretations

January 9, 2015 Acharya Tankeshwar 6

Epsilometer test (E- test ) is an ‘exponential gradient’ method of determination of antimicrobial resistance. The E-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms.This is a quantitative method that applies […]

Antibiotic Resistance

Modified Hodge Test (MHT) for Carbapenemase detection: Principle, Method and Interpretations

January 6, 2015 Acharya Tankeshwar 1

Modified Hodge Test (MHT) is a simple phenotypic test for detection of presence of carbapenemase enzyme in bacteria.  Positive MHT test is observed in Klebsiella pneumoniae carbapenemase (KPC), Metallo Beta lactamase (MBL) and the SME-1 […]

Emerging Infectious Diseases

Seven Important Facts about Ebola Virus and Ebola Disease: Every person should know

January 6, 2015 Acharya Tankeshwar 0

Ebola is one of the scariest, highly infectious and deadly diseases, ever known to Humankind. It transmits via the body fluids of a person who is sick with or had died from Ebola (blood, vomit, […]

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