Nitrate reduction test is used for the differentiation of members of Enterobacteriaceae on the basis of their ability to produce nitrate reductase enzyme that hydrolyze nitrate (NO3–) to nitrite (NO2–) which may then again be degraded […]
Hektoen Enteric (HE) Agar was introduced in 1968 by Sylvia King and William I. Metzger. They formulated HE Agar medium while working at the Hektoen Institute in Chicago, to increase the recovery Salmonella and Shigella from clinical specimens. […]
Salmonella is a member of the family Enterobacteriaceae. It is a Gram-negative bacilli, motile and non-lactose fermenter. The genus can be divided into two species (S. enterica and S. bongori), based on their phenotypic profile. […]
Principle String Test: When an isolated colony (18-24 hour growth) of a suspected bacterium is emulsified in Sodium deoxycholate or Sodium taurocholate (commonly known as bile salt),it lyses the cell wall of the bacterium releasing […]
In my undergraduate teaching class, one of the most common query of students is; “Why do people with sickle cell anemia not get malaria”? The above query is only partially right because: Sickle cell hemoglobin confers […]
Epsilometer test (E- test ) is an ‘exponential gradient’ method of determination of antimicrobial resistance. The E-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms.This is a quantitative method that applies […]
Modified Hodge Test (MHT) is a simple phenotypic test for detection of presence of carbapenemase enzyme in bacteria. Positive MHT test is observed in Klebsiella pneumoniae carbapenemase (KPC), Metallo Beta lactamase (MBL) and the SME-1 […]
Ebola is one of the scariest, highly infectious and deadly diseases, ever known to Humankind. It transmits via the body fluids of a person who is sick with or had died from Ebola (blood, vomit, […]
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