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Home2015March

Month: March 2015

Bacteriology

Voges Proskauer (VP) Test: Principle, Procedure and Results

March 17, 2015 Acharya Tankeshwar 8

Voges-Proskauer is a double eponym, named after two microbiologists working at the beginning of the 20th century.  They first observed the red color reaction produced by appropriate culture media after treatment with potassium hydroxide. It […]

Bacteriology

CLED Agar: composition, uses and typical colony characteristics

March 16, 2015 Acharya Tankeshwar 24

CLED (cysteine-, lactose-, and electrolyte-deficient) agar is a differential culture medium for use in isolating and enumerating bacteria in urine from the suspected cases of Urinary Tract Infection. CLED agar supports the growth of all […]

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Bacteriology

Decarboxylation Test:  Types, uses, principles, procedure and results

March 16, 2015 Acharya Tankeshwar 7

Decarboxylases are a group of substrate specific enzymes that are capable of reacting with the carboxyl  (COOH) portion of amino acids, forming alkaline-reacting amines and byproduct carbon dioxide.  Increased pH of the medium is detected by […]

Bacteriology

Hippurate Hydrolysis test: principle, procedure, uses and results

March 14, 2015 Acharya Tankeshwar 2

Hippurate is the glycine conjugate of benzoic acid.  When hippurate is hydrolysed by an organism glycine and benzoic acid are formed. In the classical test (48 hours), The benzoic acid produced was detected using ferric […]

Bacteriology

Lancefield grouping of clinically relevant beta-hemolytic streptococci

March 12, 2015 Acharya Tankeshwar 6

Streptococci are Gram positive cocci either diplococci (lanceolate shaped; S. pneumoniae) or in chains. They are catalase negative (An important test which differentiate it from another cocci, Staphylococci). This fastidious gram positive cocci may give […]

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Lab Diagnosis of Viral Disease

Monospot test: Principle, Procedure, Accuracy and Uses

March 10, 2015 Acharya Tankeshwar 0

Monospot test is a form of the heterophile antibody test which is used for the rapid screening of infectious mononucleosis (mono).  Epstein–Barr virus (EBV) causes the disease infectious mononucleosis. Blood, serum or plasma is taken […]

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