January 2014 - Learn Microbiology Online

Common Fungal Culture Media and their uses

January 26, 2014 Acharya Tankeshwar 10

This post was most recently updated on March 25th, 2020Two general types of culture media are essential to ensure the primary recovery of all clinically significant fungi from clinical specimens. One medium should be non-selective (such […]

Identification of Fungi based on microscopic feature (Yeasts and Molds)

January 26, 2014 Acharya Tankeshwar 11

This post was most recently updated on May 21st, 2016If you observe a probable fungus in the culture plate, colony characteristics are first assessed to determine the broad group of the isolate. Once the initial observations are […]

New York City Medium Agar: Introduction, principle, composition and uses

January 26, 2014 Acharya Tankeshwar 0

This post was most recently updated on August 26th, 2019New York City (NYC) Medium is primarily designed for isolation of pathogenic Neisseria. It also supports the growth of genital mycoplasmas (Mycoplasma hominis and Ureaplasma urealyticum). New […]

Methyl Red (MR) test: Principle, procedure and results

January 24, 2014 Acharya Tankeshwar 10

This post was most recently updated on February 1st, 2020Methyl Red (MR) test determines whether the microbe performs mixed acids fermentation when supplied glucose. Types and proportion of fermentation products produced by anaerobic fermentation of […]

Gelatin Hydrolysis Test: Principle, Procedure and expected results

January 24, 2014 Acharya Tankeshwar 6

This post was most recently updated on February 1st, 2020Gelatin is a protein derived from the animal protein collagen– component of vertebrate connective tissue. It has been used as a solidifying agent in food for a […]

Flagella Staining: Procedure and Results

January 24, 2014 Acharya Tankeshwar 1

This post was most recently updated on December 10th, 2019Most motile bacteria possess flagella, the shape, number and position of which are important characteristics in the differentiation of genera and species identification. Read more about […]

Microbiology Laboratory Safety Rules and Procedure

January 23, 2014 Acharya Tankeshwar 4

This post was most recently updated on January 26th, 2015You will find some of these rules and procedures listed in the beginning material of your laboratory manual. If you find any safety rules or procedures […]

Bile-Esculin test for Enterococcus species: Principle, Procedure and Results

January 21, 2014 Acharya Tankeshwar 6

This post was most recently updated on April 20th, 2019Bile-esculin test is widely used to differentiate Enterococci and Non-enterococcus group D streptococci, which are bile tolerant and can hydrolyze esculin to esculetin, from non-group D […]

Salt Tolerance Test for Enterococcus species: Principle, Procedure and results

January 21, 2014 Acharya Tankeshwar 5

This post was most recently updated on April 26th, 2018Salt Tolerance Test is used in the identification of enterococcal group D Streptococcus on the basis of their salt tolerance. The ability of the bacteria to […]

Principle and procedure of Cellulose Tape Preparation for Pinworm examination

January 19, 2014 Acharya Tankeshwar 1

This post was most recently updated on August 26th, 2019Principle The clear-cellulose tape preparation is the most widely used procedure for the detection of human pinworm infections. Adult Enterobius vermicularis worms inhabit the large intestine […]