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Home2014January

Month: January 2014

Culture Media used in Microbiology

Common Fungal Culture Media and their uses

January 26, 2014 Acharya Tankeshwar 10

Two general types of culture media are essential to ensure the primary recovery of all clinically significant fungi from clinical specimens. One medium should be non-selective (such as Brain Heart Infusion Agar; i.e., one that will permit […]

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Mycology

Identification of Fungi based on microscopic feature (Yeasts and Molds)

January 26, 2014 Acharya Tankeshwar 11

If you observe a probable fungus in the culture plate, colony characteristics are first assessed to determine the broad group of the isolate. Once the initial observations are made, following microscopic criteria are used to make a […]

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Bacteriology

New York City Medium Agar: Introduction, principle, composition and uses

January 26, 2014 Acharya Tankeshwar 0

New York City (NYC) Medium is primarily designed for isolation of pathogenic Neisseria.  It also supports the growth of genital mycoplasmas (Mycoplasma hominis and Ureaplasma urealyticum). New York City (NYC) medium is useful in the diagnosis […]

Biochemical tests in Microbiology

Methyl Red (MR) test: Principle, procedure and results

January 24, 2014 Acharya Tankeshwar 10

Methyl Red (MR)  test determines whether the microbe performs mixed acids fermentation when supplied glucose. Types and proportion of fermentation products produced by anaerobic fermentation of glucose are one of the key taxonomic characteristics which […]

Bacteriology

Gelatin Hydrolysis Test: Principle, Procedure and expected results

January 24, 2014 Acharya Tankeshwar 6

Gelatin is a protein derived from the animal protein collagen– component of vertebrate connective tissue. It has been used as a solidifying agent in food for a long time. Gelatin hydrolysis test is a great way to […]

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Staining techniques in Microbiology

Flagella Staining: Procedure and Results

January 24, 2014 Acharya Tankeshwar 3

Most motile bacteria possess flagella, the shape, number and position of which are important characteristics in the differentiation of genera and species identification. Read more about structure and importance of bacterial flagella clicking this link. […]

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Bacteriology

Microbiology Laboratory Safety Rules and Procedure

January 23, 2014 Acharya Tankeshwar 4

You will find some of these rules and procedures listed in the beginning material of your laboratory manual. If you find any safety rules or procedures listed here appears to be in conflict with those […]

Biochemical tests in Microbiology

Bile-Esculin test for Enterococcus species: Principle, Procedure and Results

January 21, 2014 Acharya Tankeshwar 6

Bile-esculin test is widely used to differentiate Enterococci and Non-enterococcus group D streptococci, which are bile tolerant and can hydrolyze esculin to esculetin, from non-group D viridans group streptococci, which grow poorly on bile.   […]

Bacteriology

Salt Tolerance Test for Enterococcus species: Principle, Procedure and results

January 21, 2014 Acharya Tankeshwar 5

Salt Tolerance Test is used in the identification of enterococcal group D Streptococcus on the basis of their salt tolerance. The ability of the bacteria to grow in the presence of variable amount of Sodium […]

Lab Diagnosis of Parasitic Disease

Principle and procedure of Cellulose Tape Preparation for Pinworm examination

January 19, 2014 Acharya Tankeshwar 1

Principle The clear-cellulose tape preparation is the most widely used procedure for the detection of human pinworm infections. Adult Enterobius vermicularis worms inhabit the large intestine and rectum; however, the eggs are not normally found […]

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