These posts are related to isolation and enumeration of bacteria.
Serial dilution is the standard method for estimating bacterial counts in samples. Learn the 10-fold dilution procedure, CFU/mL calculation, the 30–300 colony rule, common errors (...
How manual, digital, and automated colony counters work, how they connect to CFU/mL counts and the 30–300 rule, and how to choose the right one for your lab.
The spread plate technique distributes a diluted sample evenly across pre-dried agar to produce surface colonies for counting and isolation. Learn the procedure, CFU/mL calculation...
The MPN test estimates bacterial concentration using statistical probability across serial dilution tube patterns. Learn its three-step procedure (presumptive, confirmatory, comple...
The pour plate method mixes the bacterial inoculum with molten agar before solidification, producing colonies both within and on the surface. Learn the procedure, 30–300 count rule...
The streak plate method isolates bacteria into pure cultures by progressive dilution across an agar surface. Learn quadrant, T-streak, radiant, and continuous methods, common error...
