Information about Electrophoresis Techniques
Detailed information about Electrophoresis Techniques
Polyacrylamide gel electrophoresis (PAGE) separates proteins by size. Learn why SDS is added, why the gel has a stacking and a resolving layer, how to choose the acrylamide percent...
Cellulose acetate electrophoresis separates serum proteins and hemoglobin variants by net charge at alkaline pH. Learn the principle, why Hb S co-migrates with Hb D and Hb G, and w...
Capillary Electrophoresis: Principle and Application
Electrophoresis separates charged molecules such as proteins and DNA by moving them through a gel in an electric field. Learn the principle, the factors that control mobility, the...
Pulsed-field gel electrophoresis (PFGE) separates DNA fragments up to 10 Mb by switching the electric field between directions, forcing large molecules to reorient. Learn why pulsi...
Agarose gel electrophoresis separates DNA fragments from 100 bp to 25 kb by size. Learn the principle, how to cast and run a gel, why supercoiled plasmid runs faster than linear DN...
Counterimmunoelectrophoresis (CIE) drives antigen and antibody toward each other in an agarose gel, producing a precipitin line within an hour. Learn the principle, why a neutral a...
