Designing PCR Primers: 6 Useful Tips
PCR primer design determines whether amplification succeeds or fails. Learn the six key parameters — length, Tm, GC content, GC clamp, specificity, and secondary structure — with t...
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PCR Techniques
Information about various types of Polymerase Chain Reaction Techniques
About PCR Techniques
More detailed information about various types of Polymerase Chain Reaction Techniques
Real-time PCR (qPCR): Principles and Applications
Real-time PCR (qPCR) amplifies and quantifies DNA simultaneously using fluorescent probes. Learn SYBR Green vs TaqMan, Ct values, and clinical uses in viral load testing.
Nested PCR: Principle and Applications
Nested PCR uses two successive PCR reactions with outer and inner primer sets to maximise sensitivity and specificity. Learn its principle, applications, and contamination risk.
Multiplex PCR: Principle, Applications
Multiplex PCR amplifies multiple targets simultaneously in one reaction. Learn primer design considerations, advantages, clinical applications, and limitations in diagnostic microb...
Reverse transcriptase (RT)-PCR: Principles, Applications
RT-PCR converts RNA to cDNA using reverse transcriptase before PCR amplification. Learn one-step vs two-step methods, primer types, and clinical uses in RNA virus detection.
Polymerase Chain Reaction (PCR): Steps, Types, Applications
PCR amplifies DNA exponentially in three steps: denaturation, annealing, and extension. Learn the components, steps, types — nested, multiplex, real-time, RT-PCR and clinical appli...