Common Fungal Culture Media: Their Uses

By Acharya Tankeshwar •  Updated: 05/03/22 •  4 min read

Two general types of culture media are essential to ensure the primary recovery of all clinically significant fungi from clinical specimens. One medium should be non-selective (such as brain heart infusion agar; i.e., one that will permit the growth of virtually all clinically relevant fungi) and other media should be selective, specially tailored to isolate specific pathogenic fungi of interest.

Common fungal culture media

For optimal recovery of the fungal pathogen, a battery of media should be used, and the followings are recommended:

  1. Media with or without cyclohexamide (cycloheximide is added to inhibit the growth of rapidly growing contaminating molds.)
  2. Media with or without an antibacterial agent (chloramphenicol, gentamicin, and ciprofloxacin are commonly used antibacterial for this purpose).

Antibacterial agents are used to kill the contaminating bacterial species. If the sample is taken from a sterile site, it is not necessary to use media containing antibacterial agents.

Common Fungal Culture Media

Fungal Culture MediaEssential ingredientsIntended use
Birdseed agarGuizotia abyssinica seeds, glucose, creatinine, chloramphenicol, monopotassium phosphate, agarSelective and differential isolation of Cryptococcus neoformans from clinical specimens.
Brain-heart infusion agarBrain heart infusion, glucose, L-cysteine hydrochloride, agarGrowth of fastidious pathogenic fungi such as Histoplasma capsulatum and Blastomyces dermatitidis.
CHROMagar Candida mediumPeptone, glucose, chloramphenicol, ‘Chromogenic ix’, agarSelective and differential chromogenic medium for the isolation and identification of various Candida species
Cornmeal agar/ Cornmeal tween agarCornmeal, Tween 80, agarIdentification of C. albicans by microscopic morphology or chlamydospore production.
Czapek-Dox agarNaNO3, K2HO4, KCl, MgSO4, FeSO4, glucose, agarIdentification of Aspergillus and Penicillium species. It can also be used for chlamydospore production by Candida albicans.
Dermatophyte test medium (DTM)Soy peptone, dextrose, cycloheximide, chloramphenicol, gentamicin sulfate, phenol red, agarPrimary and differential fungal cultures medium to isolate and identify dermatophytes.
Inhibitory mold agar (IMA)Tryptone, beef extract, yeast extract, starch, dextrin, chloramphenicol, gentamicin, and saline bufferPrimary recovery of pathogenic fungi exclusive of dermatophytes.
Mycosel/mycobiotic agarPapaic digest of soybean meal, dextrose, cycloheximide, chloramphenicol, agarHighly selective medium; recommended for the isolation of pathogenic fungi from materials containing a large amount of fungal and bacterial flora
Potato dextrose agarPotato extract, potato infusion, dextrose, agarIdeal medium for slide culture preparation. It promotes sporulation of dermatophytes. Demonstration of pigment production by T. rubrum.
Potato flake agarPotato flakes, dextrose, agarPrimary recovery of saprophytic and pathogenic fungi.
Rice starch agarCream of rice, Tween 80, agarProduction of chlamydospore in Candida albicans
Sabouraud dextrose agarPancreatic digest of casein, glucose, agarGrowth and maintenance of clinically important fungal pathogens.

Brain-heart infusion (BHI) agar

It is a non-selective fungal culture medium that permits the growth of virtually all clinically relevant fungi. It is used for the primary recovery of saprophytic and dimorphic fungi.

Czapek dox agar

Czapek dox agar is a semisynthetic medium containing sodium nitrate as the sole source of nitrogen. It is used for the subculture of Aspergillus species for their differential diagnosis.

Inhibitory mold agar (IMA)

Inhibitory mold agar (IMA) is an enriched medium containing chloramphenicol (with some formulations containing gentamicin).  It supports the growth of a wide range of fungi and, due to its antibiotic content, may inhibit bacterial growth. It is used for the primary recovery of dimorphic pathogenic fungi. Saprophytic fungi and dermatophytes will not be recovered.

Mycosel/Mycobiotic agar

Niger Seed Agar

It is used for the identification of Cryptococcus neoformans.

Potato Dextrose Agar (PDA)

Potato Dextrose Agar (PDA) is a common fungal growth media made from an infusion of potato and dextrose. It can be supplemented with acid or antibiotics to inhibit bacterial growth. It is recommended for plate count methods for microbial testings of foods, dairy products, and cosmetics.

Sabouraud’s Heart Infusion (SABHI) agar

Primary recovery of saprophytic and dimorphic fungi, particularly fastidious strains.

Penicillium notatum on Sabouraud agar Image source: ASM
Penicillium notatum on Sabouraud agar
Image source: ASM

Sabouraud’s dextrose agar (SDA)

Potato flake agar

Primary recovery of saprophytic and dimorphic fungi, particularly fastidious and slow-growing strains.

Further reading and sources: For more information please read the books:

  1. Koneman’s color atlas and textbook of diagnostic microbiology and
  2. Bailey and Scott’s Diagnostic Microbiology

Acharya Tankeshwar

Hello, thank you for visiting my blog. I am Tankeshwar Acharya. Blogging is my passion. As an asst. professor, I am teaching microbiology and immunology to medical and nursing students at PAHS, Nepal. I have been working as a microbiologist at Patan hospital for more than 10 years.

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7 responses to “Potato Dextrose Agar (PDA): Principle, Composition, Colony Characteristics”

  1. Alfred says:

    I am working to identify a pathogen in the lab. I cultured the samples (leaves) on PDA and there was a fungi growth but some of the plates appears to contain bacteria. Please I want to know if it is possible for bacteria to grow on PDA, if not contamination.
    Thank you.

    • HAGENIMANA Alexandre says:

      i do think there was the contamination of your plate another posibility is that u did not use tartaric acid to inhibit bacterial growth.

  2. K.meena says:

    Wheather PDA basal medium is suitable for the growth of Mucor species which are present in soil.

  3. K.meena says:

    Wheather PDA basal medium is suitable for the growth of Mucor species of soil

  4. John Meomikem Ehiobu says:

    Pls to check bacteria growth, in raising a pure culture of fungi,
    i will like to know the quantity of antibiotic prepared and added to each plate of pda

  5. ahmed says:

    how to make spore suspension from fungal or bacterial culture? thanks for help

  6. Nguyen Thu Ha says:

    I would like to enumeration of Bacillus from soils. How to do that?
    Thanks for help.

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