Last updated on May 30th, 2021Real-time PCR also called quantitative PCR (qPCR) is a variant of standard polymerase chain reaction in which amplification and simultaneous quantitation of a target DNA is done in the same […]
Last updated on June 21st, 2021Nested PCR is a modification of PCR designed to increase the sensitivity and specificity of the assay reaction. It involves the use of two primer sets directed against the same […]
Last updated on July 4th, 2021Multiplex PCR is a variant of PCR method in which more than one target sequence are amplified using multiple sets of primers within a single PCR mixture. This enables amplification […]
Last updated on May 30th, 2021Reverse Transcriptase PCR (RT-PCR) is a variation of the polymerase chain reaction that amplifies target RNA. Addition of reverse transcriptase (RT) enzyme prior to PCR makes it possible to amplify […]
Last updated on July 22nd, 2021Polymerase chain reaction (PCR) is an efficient and cost-effective molecular tool to copy or amplify small segments of DNA or RNA. PCR combines the principles of complementary nucleic acid hybridization […]
Copyright © microbeonline
