Types of PCR

Real-time PCR: Principles and Applications

Real-time PCR: Principles and Applications

Amplification and simultaneous quantitation of a target DNA is done in same PCR machine and accumulation of amplicon is monitored in real-time.

Nested PCR: Principle and Applications

Nested PCR: Principle and Applications

Two primer sets (outer primer and inner or nested primer) directed against the same target are used to amplify the target gene sequence.

Multiplex PCR: Principle, Applications

Multiplex PCR: Principle, Applications

In multiplex PCR, more than one target sequence are amplified using multiple sets of primers within a single PCR mixture.

Reverse transcriptase (RT)-PCR: Principles, Applications

Reverse transcriptase (RT)-PCR: Principles, Applications

In reverse-transcriptase PCR, RNA is first reverse-transcribed into cDNA, which is then used as the template for second PCR amplification.

Polymerase Chain Reaction (PCR):  Steps, Types, Applications

Polymerase Chain Reaction (PCR):  Steps, Types, Applications

Molecular technology to amplify a single or few copies of a piece of DNA to generate millions copies of DNA.