Nested PCR is a modification of PCR designed to increase the sensitivity and specificity of the assay reaction. It involves the use of two primer sets directed against the same target and two successive PCR […]
Multiplex PCR is a variant of PCR method in which more than one target sequence is amplified using multiple sets of primers within a single PCR mixture. This enables amplification of several gene segments at […]
Reverse Transcriptase PCR (RT-PCR) is a variation of the polymerase chain reaction that amplifies target RNA. Addition of reverse transcriptase (RT) enzyme prior to PCR makes it possible to amplify and detect RNA targets. Reverse […]
This post was most recently updated on December 26th, 2019Polymerase chain reaction (PCR) is an efficient and cost-effective molecular tool to copy or amplify small segments of DNA or RNA. PCR combines the principles of […]
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