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Tag: Types of PCR

link to Designing PCR Primers: 6 Useful Tips

Designing PCR Primers: 6 Useful Tips

Designing a good pair of PCR primers is probably the single most important factor for successful PCR reactions. A primer is a short, synthetic, single-stranded DNA sequence that is complementary to...

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link to Real-time PCR: Principles and Applications

Real-time PCR: Principles and Applications

Real-time PCR also called quantitative PCR (qPCR), is a variant of standard polymerase chain reaction in which amplification and simultaneous quantitation of a target DNA is done in the same PCR...

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link to Nested PCR: Principle and Applications

Nested PCR: Principle and Applications

Nested PCR is a modification of PCR designed to increase the sensitivity and specificity of the assay reaction. It involves the use of two primer sets directed against the same target and two...

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link to Multiplex PCR: Principle, Applications

Multiplex PCR: Principle, Applications

Multiplex PCR is a variant of PCR methods in which more than one target sequence are amplified using multiple sets of primers within a single PCR mixture. This enables amplification of several gene...

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link to Reverse transcriptase (RT)-PCR: Principles, Applications

Reverse transcriptase (RT)-PCR: Principles, Applications

Reverse Transcriptase PCR (RT-PCR) is a variation of the polymerase chain reaction that amplifies target RNA. Addition of reverse transcriptase (RT) enzyme prior to PCR makes it possible to amplify...

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link to Polymerase Chain Reaction (PCR):  Steps, Types, Applications

Polymerase Chain Reaction (PCR):  Steps, Types, Applications

Polymerase chain reaction (PCR) is an efficient and cost-effective molecular tool to copy or amplify small segments of DNA or RNA.  PCR combines the principles of complementary nucleic acid...

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