Amplification and simultaneous quantitation of a target DNA is done in same PCR machine and accumulation of amplicon is monitored in real-time.
Two primer sets (outer primer and inner or nested primer) directed against the same target are used to amplify the target gene sequence.
In multiplex PCR, more than one target sequence are amplified using multiple sets of primers within a single PCR mixture.
In reverse-transcriptase PCR, RNA is first reverse-transcribed into cDNA, which is then used as the template for second PCR amplification.
Molecular technology to amplify a single or few copies of a piece of DNA to generate millions copies of DNA.
