Amies transport medium is a widely used and effective semisolid medium for the transportation of swab specimens to the microbiology laboratory. Placing swabs in a moist container or transport medium prevents drying and death of […]
Culture media play a pivotal role in any microbiology laboratory for isolation, identification and sensitivity testing of different pathogens. Most of the laboratories usually prepare their own media or purchase prepared media for routine diagnostics […]
Brain Heart Infusion (BHI) broth is a general-purpose liquid medium used for the cultivation and maintenance of a wide variety of fastidious and non-fastidious microorganisms, including aerobic and anaerobic bacteria, yeast and molds from a […]
Chromogenic Culture Media are culture medium used to isolate, identify, and differentiate specific microorganism from a heterogeneous population. The medium contains chromogenic substrate which is utilized by the microorganism to give colored colonies that is specific […]
Cary-Blair transport medium can be used to transport of clinical specimens suspected to contain enteric pathogens, including Shigella, Salmonella, Vibrio cholerae, and Escherichia coli O157:H7. Since this transport medium has a high pH (8.4), viability […]
Alkaline peptone water (APW) is recommended as an enrichment broth for isolating Vibrio cholerae from clinical samples and non clinical samples (suspected food & water samples). A number of other broth media have been described for enrichment of […]
Pseudosel Agar or Cetrimide Agar is used for the selective isolation and presumptive identification of Pseudomonas aeruginosa . The medium was first developed by Lowburry and is a modification of Tech Agar (developed by King […]
CLED (cysteine-, lactose-, and electrolyte-deficient) agar is a differential culture medium for use in isolating and enumerating bacteria in urine from the suspected cases of Urinary Tract Infection. CLED agar supports the growth of all […]
Thiosulfate citrate bile salts sucrose (TCBS) agar is a selective as well as differential culture medium used for selective isolation of Vibrio spp from a variety of clinical and nonclinical specimens. Vibrio cholerae is the […]
Chocolate Agar (CAP) is the lysed blood agar. The name is itself derived from the fact that red blood cell (RBC) lysis gives the medium a chocolate-brown color. Chocolate agar is used for the isolation […]
Buffered charcoal yeast extract agar (BCYE) was developed by Feeley et al and then further modified by Edelstein for selective cultivation of Legionella species. BCYE Agar is an enrichment medium that is used for the […]
Eosin Methylene Blue (EMB) agar is both selective and differential culture medium. It is a selective culture medium for Gram-negative bacteria (selects against Gram-positive bacteria) and is commonly used for the isolation and differentiation of […]
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