Danish physician Hans Christian Gram developed the Gram staining method in 1884. Gram staining procedure uses four chemicals; crystal violet, iodine, alcohol, and safranin, to stain...
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Catalase is an enzyme produced by microorganisms that live in oxygenated environments to neutralize the bactericidal effects of toxic forms of oxygen metabolites such as hydrogen peroxide (H2O2). The...
Blood agar is an enriched bacterial growth medium. Fastidious organisms, such as streptococci, do not grow well on ordinary growth media but grow on blood agar. Blood agar is a growth medium with...
MacConkey agar (MAC) is a selective and differential bacterial culture media. MacConkey medium is selective for gram-negative bacteria and differentiates the gram-negative bacteria based on...
Triple Sugar Iron (TSI) Agar: Principle, Results, and Interpretation
Whenever you see the name of this test i.e. 'Triple Sugar Iron Agar', you have to remember that it's a test that has three sugar (lactose, sucrose, and glucose) and also iron; and it contains...
Defined media and complex media are two broad classes of culture media used in microbiology. Defined media are prepared by adding precise amounts of highly purified inorganic or organic chemicals to...
