Mycobacterium tuberculosis (MTB) is the causative agent of tuberculosis (TB) in humans. It is a slightly curved rod arranged singly or in groups. Mycobacterium tuberculosis grows very slowly, taking up to 6 weeks for visible […]
M. tuberculosis requires aerobic conditions and a protein-enriched medium for culture. Löwenstein–Jensen (LJ) slopes are the main solid media used to culture Mycobacteria. It contains inspissated eggs, malachite green, and glycerol (or pyruvate). LJ medium […]
GeneXpert MTB/RIF assay is a rapid diagnosis test of Tuberculosis (TB) and drug resistance. It is revolutionizing TB control with aids in prompt diagnosis and treatment (selection of appropriate TB regimen). GeneXpert MTB/RIF assay is […]
The Mantoux tuberculin skin test is the standard method of determining whether a person is infected with Mycobacterium tuberculosis or have exposure to tubercle bacilli. It was developed by Koch in 1890 but the intradermal […]
Mycobacteria are distinguished from other micro-organisms by thick lipid-containing cell-walls that retain biochemical stains despite decolourisation by acid-containing reagents (so-called ‘acid-fastness’). Advantages: Microscopy of sputum smears is simple and inexpensive, quickly detecting infectious cases of pulmonary […]
Ziehl-Neelsen (ZN) method of Acid Fast staining technique is used to stain Mycobacterium species including M. tuberculosis, M. ulcerans, and M. leprae and nontuberculous mycobacteria (NTM). Detection of acid-fast bacilli (AFB) in stained and acid-washed […]
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