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HomeMycobacterium tuberculosis

Mycobacterium tuberculosis

laboratory-diagnosis of Mycobacterium tuberculosis infection
Bacteriology

Laboratory Diagnosis of Mycobacterium tuberculosis Infection

May 26, 2020 Nisha Rijal 0

Mycobacterium tuberculosis (MTB) is the causative agent of tuberculosis (TB) in humans. It is a slightly curved rod arranged singly or in groups. Mycobacterium tuberculosis grows very slowly, taking up to 6 weeks for visible […]

Bacteriology

Löwenstein–Jensen (LJ) Medium: Prepartion and Uses

April 29, 2016 Acharya Tankeshwar 6

M. tuberculosis requires aerobic conditions and a protein-enriched medium for culture. Löwenstein–Jensen (LJ) slopes are the main solid media used to culture Mycobacteria. It contains inspissated eggs, malachite green, and glycerol (or pyruvate). LJ medium […]

GeneXpert MTB/RIF Assay
Bacteriology

GeneXpert MTB/RIF Assay: Principle, Procedure, Results and Interpretations

January 4, 2016 Acharya Tankeshwar 17

GeneXpert MTB/RIF assay is a rapid diagnosis test of Tuberculosis (TB) and drug resistance.  It is revolutionizing TB control with aids in prompt diagnosis and treatment (selection of appropriate TB regimen). GeneXpert MTB/RIF assay is […]

Mantoux tuberculin skin test
Bacteriology

Tuberculin Skin Test (Mauntoux test):Principle, Procedure, Results and Limitations

August 22, 2015 Nisha Rijal 3

The Mantoux tuberculin skin test is the standard method of determining whether a person is infected with Mycobacterium tuberculosis or have exposure to tubercle bacilli. It was developed by Koch in 1890 but the intradermal […]

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Bacteriology

Advantage, Disadvantage and limitations of Microscopy to detect Acid Fast Bacilli (AFB)

December 6, 2013 Acharya Tankeshwar 0

Mycobacteria are distinguished from other micro-organisms by thick lipid-containing cell-walls that retain biochemical stains despite decolourisation by acid-containing reagents (so-called ‘acid-fastness’). Advantages: Microscopy of sputum smears is simple and inexpensive, quickly detecting infectious cases of pulmonary […]

Acid fast bacilli
Bacteriology

Ziehl-Neelsen technique (AFB Staining): Principle, Procedure and reporting

December 6, 2013 Acharya Tankeshwar 27

Ziehl-Neelsen (ZN) method of Acid Fast staining technique is used to stain Mycobacterium species including M. tuberculosis, M. ulcerans, and M. leprae and nontuberculous mycobacteria (NTM).  Detection of acid-fast bacilli (AFB) in stained and acid-washed […]

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