Generally, when people (either general public or a physician or laboratory personnel) listen/read the term “antibiotic resistance” they think that, a bacterium which was previously sensitive to a particular antibacterial agent has now developed resistance […]
The term “blotting” refers to the transfer of biological samples from a gel to a membrane and their subsequent detection on the surface of the membrane. Southern blot is used for transferring DNA, Northern blot […]
Streptococcus pneumoniae is one of the most common causes of community-acquired pneumonia (CAP). Pneumococcal pneumonia has a mortality rate of > 30%, depending on bacteremia, age and underlying diseases. When not properly diagnosed and treated, S. […]
Rapid diagnostic tests or RDTs for Malaria are a way to test whether a person with malaria like symptoms actually has malaria. Malaria parasites produce proteins called antigens. RDTs detect these malarial antigens in a person’s […]
The carbohydrate fermentation test is used to determine whether or not bacteria can ferment a specific carbohydrate. Carbohydrate fermentation patterns are useful in differentiating among bacterial groups or species. It tests for the presence of acid and/or […]
Robertson’s Cooked Meat (RCM) medium is used for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species. It is also known as Cooked Meat Broth (CMB) as it contains pieces of fat free […]
Phenylalanine deaminase test also known as phenylpyruvic acid (PPA) test is used to test the ability of an organism to produce enzyme deaminase. This enzyme removes the amine group from the amino acid phenylalanine and […]
Microorganisms can produce different types of volatile compounds which may give characteristics smell, pleasant scent or pungent odor. Production of these volatile chemicals depends on the metabolic characteristics of that particular organism. Sometimes scent or […]
Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. In this method, fixed amount of inoculum (generally 1 ml) from a broth/sample is […]
Capsule stain is a type of differential stain which uses acidic and basic dyes to stain background & bacterial cells respectively so that presence of capsule is easily visualized. Capsule is synthesized in the cytoplasm […]
Thioglycollate broth is the enrichment broth most frequently used in diagnostic bacteriology. This broth supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms. It contains many nutritive factors, including casein, yeast, beef extracts, and vitamins […]
Urea breath test (UBT) is a qualitative and non-invasive rapid test for the diagnosis of Helicobacter pylori (the major cause of gastric and duodenal ulcers) infections. Helicobacter pylori is a fastidious bacterium which is known to be a potent producer of […]
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