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Immunology

Western Blot Technique: Principle, Procedures and Uses

May 12, 2017 Bina Bhandari 6

The term “blotting” refers to the transfer of biological samples from a gel to a membrane and their subsequent detection on the surface of the membrane. Southern blot is used for transferring DNA, Northern blot […]

Binax Now Streptococcus Pneumoniae Test
Bacteriology

Pneumococcal Urinary Antigen Testing (UAT): Principle, Procedure and Results  

February 24, 2017 Acharya Tankeshwar 0

Streptococcus pneumoniae is one of the most common causes of community-acquired pneumonia (CAP).  Pneumococcal pneumonia has a mortality rate of > 30%, depending on bacteremia, age and underlying diseases.  When not properly diagnosed and treated, S. […]

Lab Diagnosis of Parasitic Disease

RDTs for Malaria Diagnosis: Principle, Results and Advantages

January 23, 2017 Acharya Tankeshwar 1

Rapid diagnostic tests or RDTs for Malaria are a way to test whether a person with malaria like symptoms actually has malaria. Malaria parasites produce proteins called antigens. RDTs detect these malarial antigens in a person’s […]

Bacteriology

Carbohydrate Fermentation Test: Uses, Principle, Procedure and Results

December 10, 2016 Acharya Tankeshwar 12

The carbohydrate fermentation test is used to determine whether or not bacteria can ferment a specific carbohydrate. Carbohydrate fermentation patterns are useful in differentiating among bacterial groups or species. It tests for the presence of acid […]

Anaerobic Bacteriology

Robertson’s Cooked Meat (RCM) Medium: Principle, Composition, Procedure and Uses

November 29, 2016 Acharya Tankeshwar 2

Robertson’s Cooked Meat (RCM) medium is used for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species.   It is also known as Cooked Meat Broth (CMB) as it contains pieces of fat-free minced […]

Bacteriology

Phenylalanine Deaminase Test : Principle, Procedure, Results and Uses

November 4, 2016 Acharya Tankeshwar 2

Phenylalanine deaminase test also known as phenylpyruvic acid (PPA) test is used to test the ability of an organism to produce enzyme deaminase.  This enzyme removes the amine group from the amino acid phenylalanine and […]

Anaerobic Bacteriology

Microbes with Characteristics Smell (good and bad)

October 17, 2016 Acharya Tankeshwar 4

Microorganisms can produce different types of volatile compounds that may give characteristics smell, pleasant scent, or pungent odor. Production of these volatile chemicals depends on the metabolic characteristics of that particular organism. Sometimes scent or […]

Bacteriology

Pour plate Method: Principle, Procedure, Uses, and (Dis) Advantages

October 16, 2016 Acharya Tankeshwar 15

Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen.  In this method, fixed amount of inoculum (generally 1 ml) from a broth/sample is […]

Bacteriology

Capsule Stain: Principle, Procedure and Results

October 15, 2016 Acharya Tankeshwar 9

Capsule stain is a type of differential stain which uses acidic and basic dyes to stain background & bacterial cells respectively so that presence of capsule is easily visualized.  Capsule is synthesized in the cytoplasm […]

Anaerobic Bacteriology

Thioglycollate broth: Principle, Composition, Preparation and Uses

October 6, 2016 Nisha Rijal 8

Thioglycollate broth is the enrichment broth most frequently used in diagnostic bacteriology. This broth supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms.  It contains many nutritive factors, including casein, yeast, beef extracts, and vitamins […]

Bacteriology

Urea Breath Test (UBT) for H.Pylori: Principle, Procedure and Results

October 3, 2016 Acharya Tankeshwar 1

Urea breath test (UBT) is a qualitative and non-invasive rapid test for the diagnosis of Helicobacter pylori (the major cause of gastric and duodenal ulcers) infections. Helicobacter pylori is a fastidious bacterium which is known to be a potent producer of […]

Immunology

The Vaccine Schedule: How Is It Determined And Why Is It Important?

September 24, 2016 Acharya Tankeshwar 0

A vast array of childhood infections can be prevented by starting immunization early and sticking closely to the vaccination schedule laid out by the below childhood vaccination checklist created by Carrington.edu. This carefully researched schedule removes all doubt […]

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    Antigen Testing for COVID-19: Principle, Procedure, Results and Interpretations
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    mRNA Vaccine: What it is and How it works?
    November 19, 2020 4
  • Francisella tularensis basic feature
    Francisella tularensis: Properties, Pathogenesis, and Laboratory Diagnosis
    October 13, 2020 0
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    Burkholderia pseudomallei: Properties, Pathogenesis and Laboratory Diagnosis
    October 9, 2020 1

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