Fungal Staining Methods

Calcofluor White Staining: Principle, Procedure, and Application

Calcofluor White Staining: Principle, Procedure, and Application

Calcofluor white staining uses fluorescent dye, which especially stains the chitin present in the cell wall of fungi.

Slide Culture for Fungi: Principle, Procedure, Results

Slide Culture for Fungi: Principle, Procedure, Results

Slide-culture is a rapid method of preparing fungal colonies for examination and identification with little disturbance as possible.

KOH Mount: Principle, Procedure, Results, Uses  

KOH Mount: Principle, Procedure, Results, Uses  

KOH preparation is used for the rapid detection of fungal elements in clinical specimens but it may not necessarily identify the species of the fungi.

Lactophenol Cotton Blue (LPCB) Mounts

Lactophenol Cotton Blue (LPCB) Mounts

Lactophenol cotton blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi.